Reagents and instruments for immunology, cell biology and molecular biology.

Lipopolysaccharides, from E. coli O55:B5

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Cat# HY-D1056-5mg

Size : 5mg

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Phone : +1 850 650 7790

Lipopolysaccharides, from E. coli O55:B5 is an endotoxin extracted from E. coli O55:B5, consisting of an antigen-specific chain, A core oligosaccharide, and lipid A. Lipopolysaccharides, from E. coli O55:B5 activates TLR-4 of immune cells. Lipopolysaccharides, from E. coli O55:B5 can induce the change of body temperature in rats with dose and serotype specificity. Lipopolysaccharides, from E. coli O55:B5 caused a heterogeneous and dose-independent increase in body temperature in rats.

For research use only. We do not sell to patients.

Lipopolysaccharides, from E. coli O55:B5 Chemical Structure

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Based on 231 publication(s) in Google Scholar

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: Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 May 23;e2105650. [Abstract]; Western blot analysis of the phenotypic markers in LPS (1 µg/mL; 24 h)-stimulated macrophages.

: Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 May 23;e2105650. [Abstract]; In LPS (1 µg/mL; 24 h)-stimulated macrophages, Representative fluorescence images of macrophage phenotypes after incubation with different pretreated neutrophils; iNOS (red), CD206 (green), and nuclei (blue).

: Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Biomater Res. 2022 Apr 25;26(1):15. [Abstract]; HUVECs ae induced with LPS 100 ng/mL for 24 h, then treated with PLCL-N, MPSS-loaded with 0.2 mg, 0.4 mg, 0.6 mg, 0.8 mg and 1 mg for another 24 h.

: Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jan 21;13(1):73. [Abstract]; Effect of NKAP on the U87MG cell sensitivity to Erastin (10 μM), iFSP1 (100 μM), SAS (500 μM), Rotenone (2.5 μM), 17-DMAG (300 nM), Staurosporine (1.5 μM), TMZ (200 μM), β-lapachone (2 μM), H2O2 (1‰), LPS (200 μg/mL), and Rapamycin (300 nM). All drug treatments are for 24 h.

: Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Sens Actuators B Chem. 15 December 2022, 132707.; LPS is used as an external stimulus to induce •OH generation in the A549 and HeLa cell lines. Co-localization fluorescence imaging in A549 and HeLa cells using MTG and PY. Cells were incubated with LPS (10.0 μg/mL) for 24 h and then stained with MTG (500 nM) and PY (5.0 μM) for 30 min.

: Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Elife. 2022 May 4;11:e76707. [Abstract]; qPCR analysis for the expression of indicated genes in D40 fVBOrs treated with lipopolysaccharide (LPS) (500 ng/mL; for 72 hr) without or with PLX5622 2 μM using DMSO as vehicle control.

: Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 Jun 10;13:859806. [Abstract]; BMDMs are planted on XF24-well seahorse plates at the density of 5 × 104 cells per well and stimulated with 100 ng/mL LPS and 20 ng/mL hIFN-γ for 48 hours.

: Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 10 March 2022.; The effect of CaNP on BMDM recruitment of T cells. Left panel: BMDM-M2 treated with CaNP (35 µg/mL) and LPS (50 ng/mL)/IFN-γ (20 ng/mL) for 48 h are in the lower compartment. CD8+ T cells are in the upper compartment. Right panel: representative images of BMDM recruitment of T cells. CD8+ T cells were labeled with FITC.

: Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: J Med Virol. 2020 Aug 10. [Abstract]; The results show Apigenin significantly decreased 3pRNA‐ but not poly I:C‐ and LPS (0.5 μg/mL)‐induced A549 cell death.

: Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: J Control Release. 2020 Jan 28;320:304-313. [Abstract]; Cells were differentiated to macrophages with PMA and then treated with 1 μg/mL LPS for 3 h.

Description

IC₅₀ & Target

TLR4

In Vitro

Lipopolysaccharides (10–80 μg/mL) selectively decreases THir (tyrosine hydroxylase immunoreactive) cells and increases culture media levels of interleukin1β (IL-1β) and tumor necrosis factor-α (TNF-α) as well as nitrite (an index of nitric oxide (NO) production)^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Solvent & Solubility

In Vitro:

H₂O : 5 mg/mL (Need ultrasonic)

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Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.43 mg/mL; Clear solution

This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 8.33 mg/mL; Clear solution; Need ultrasonic and warming and heat to 60°C

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

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SDS (251 KB)

COA (300 KB)

Handling Instructions (2659 KB)

References

[1]. Kabanov DS, et al. Structural analysis of lipopolysaccharides from Gram-negative bacteria. Biochemistry (Mosc). 2010 Apr;75(4):383-404. [Content Brief]

[2]. Cai KC, et al. Age and sex differences in immune response following LPS treatment in mice. Brain Behav Immun. 2016 Nov;58:327-337. [Content Brief]

[3]. Heinrichs DE, et al. Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica. Mol Microbiol. 1998 Oct;30(2):221-32. [Content Brief]

[4]. Ying Liu, et al. Podocyte-Released Migrasomes in Urine Serve as an Indicator for Early Podocyte Injury. Kidney Dis (Basel). 2020 Nov;6(6):422-433. [Content Brief]

[5]. Gayle DA, et al. Lipopolysaccharide (LPS)-induced dopamine cell loss in culture: roles of tumor necrosis factor-alpha, interleukin-1beta, and nitric oxide. Brain Res Dev Brain Res. 2002 Jan 31;133(1):27-35. [Content Brief]

[6]. Ren Q, Guo F, Tao S, Huang R, Ma L, Fu P. Flavonoid fisetin alleviates kidney inflammation and apoptosis via inhibiting Src-mediated NF-κB p65 and MAPK signaling pathways in septic AKI mice. Biomed Pharmacother. 2020 Feb;122:109772. [Content Brief]

[7]. Koc F, Tekeli MY, Kanbur M, Karayigit MÖ, Liman BC. The effects of chrysin on lipopolysaccharide-induced sepsis in rats. J Food Biochem. 2020 Sep;44(9):e13359. [Content Brief]

[8]. Xiao Z, Kong B, Fang J, Qin T, Dai C, Shuai W, Huang H. Ferrostatin-1 alleviates lipopolysaccharide-induced cardiac dysfunction. Bioengineered. 2021 Dec;12(2):9367-9376. [Content Brief]

[9]. Dogan M D,et al. Effects of different serotypes of Escherichia coli lipopolysaccharides on body temperature in rats[J]. Life sciences, 2000, 67(19): 2319-2329.

[1]. Kabanov DS, et al. Structural analysis of lipopolysaccharides from Gram-negative bacteria. Biochemistry (Mosc). 2010 Apr;75(4):383-404.

[2]. Cai KC, et al. Age and sex differences in immune response following LPS treatment in mice. Brain Behav Immun. 2016 Nov;58:327-337.

[3]. Heinrichs DE, et al. Molecular basis for structural diversity in the core regions of the lipopolysaccharides of Escherichia coli and Salmonella enterica. Mol Microbiol. 1998 Oct;30(2):221-32.

[4]. Ying Liu, et al. Podocyte-Released Migrasomes in Urine Serve as an Indicator for Early Podocyte Injury. Kidney Dis (Basel). 2020 Nov;6(6):422-433.

[5]. Gayle DA, et al. Lipopolysaccharide (LPS)-induced dopamine cell loss in culture: roles of tumor necrosis factor-alpha, interleukin-1beta, and nitric oxide. Brain Res Dev Brain Res. 2002 Jan 31;133(1):27-35.

[6]. Ren Q, Guo F, Tao S, Huang R, Ma L, Fu P. Flavonoid fisetin alleviates kidney inflammation and apoptosis via inhibiting Src-mediated NF-κB p65 and MAPK signaling pathways in septic AKI mice. Biomed Pharmacother. 2020 Feb;122:109772.

[7]. Koc F, Tekeli MY, Kanbur M, Karayigit MÖ, Liman BC. The effects of chrysin on lipopolysaccharide-induced sepsis in rats. J Food Biochem. 2020 Sep;44(9):e13359.

[8]. Xiao Z, Kong B, Fang J, Qin T, Dai C, Shuai W, Huang H. Ferrostatin-1 alleviates lipopolysaccharide-induced cardiac dysfunction. Bioengineered. 2021 Dec;12(2):9367-9376.

[9]. Dogan M D,et al. Effects of different serotypes of Escherichia coli lipopolysaccharides on body temperature in rats[J]. Life sciences, 2000, 67(19): 2319-2329.