Maclura pomifera (MPA) (AP)

Pure Maclura pomifera lectin (MPA) from Osage Orange, Alkaline Phosphatase conjugated.||Lectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. ||Lectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.||Carbohydrate Specificity: Melibiose [Gal apha(1,6) Glc]>alpha-D-Galactose.||Inhibitory Carbohydrate: Not inhibited by simple sugars at < 50mM. Lactose and Sialic acid|will exhibit some inhibitory potency at concentrations greater than 75mM. Sialyllactose alpha (2, 6) is the most potent inhibitor of leukoagglutination.||Activity: Less than 5ug/ml will agglutinate type O human erythrocytes. Less than 0.1ug/ml will agglutinate neuraminidase treated cells.||Chemical Used for Conjugation: Alkaline Phosphatase.||Storage and Stability: |May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.|Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.