MAP Kinase p38 alpha (Mitogen-activated Protein Kinase p38 alpha, MAPK p38 alpha, CRK1, Csbp1, Csbp2, Mitogen-activated Protein Kinase 14, MAP Kinase 14, MAPK 14, Mapk14, Mxi2, p38, p38alpha, p38-alpha, p38a, p38MAPK, PRKM14, PRKM15) (Biotin)

Cat# M2352-03-Biotin-100ul

Size : 100ul

Brand : US Biological

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M2352-03-Biotin MAP Kinase p38 alpha (Mitogen-activated Protein Kinase p38 alpha, MAPK p38 alpha, CRK1, Csbp1, Csbp2, Mitogen-activated Protein Kinase 14, MAP Kinase 14, MAPK 14, Mapk14, Mxi2, p38, p38alpha, p38-alpha, p38a, p38MAPK, PRKM14, PRKM15) (Biotin)

Clone Type
Polyclonal
Host
mouse
Source
mouse
Isotype
IgG2a,k
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hm Hu Mk Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Recent studies on mitogen-activated protein kinases (MAPKs) have uncovered the presence of at least three distinct MAP kinase pathways in mammalian cells (1). These MAP-kinase cascades are utilized by the cell for related yet distinct signaling pathways, thereby functioning as separate modules containing sequentially acting signaling elements. Each MAPK module consists minimally of three protein kinases: a MAPK kinase kinase (MEKK), a MAPK kinase (MEK or MKK), and a MAPK. The terminal MAPKs components of the three cascades are grouped as follows: 1. ERKs-extracellular signal regulated kinases. 2. JNKs/SAPKs-c-Jun amino terminal kinases/ stress-activated protein kinase. 3. p38/HOG-related to the yeast HOG1 protein and also referred to as CSBP1&2, RK, or Mpk2. As suggested by their name, the ERKs are regulated predominantly by extracellular ligands which act though transmembrane receptors with intrinsic or associated tyrosine kinase activity. On the other hand, the JNKs and p38 are activated by pro-inflammatory cytokines and environmental stress such as osmotic shock, and UV light. One important feature shared by the ERK, JNK and p38 kinases is the presence of the regulatory motif Thr-X-Tyr. Each kinase group, however, possess a distinct amino acid in the central (X) position: ERKs have a glutamic acid (Glu), JNKs have a proline (Pro), and p38 has a glycine (Gly). Phosphorylation of both the conserved threonine and tryosine residues by a dual specificity MEK (MKK) is required for kinase activation. Once activated, the MAP kinases phosphorylate their substrates on serine and/or threonine residues with consequent effect on the substrate’s function and/or activity. The p38 (CSBP, RK, or Mpk2) protein is the newest member of the MAPK family to be isolated (1-12). ||Applications: |Suitable for use in ELISA and Western Blot. Other applications not tested.||Recommended Dilution:|ELISA: 0.1-1ug/ml|Western Blot: 1ug/ml ||Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a,k|Clone No: 3G180|Host: mouse|Source: mouse|Concentration: As reported|Form: Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.|Purity: Purified by Protein A affinity chromatography.|Immunogen: Full-length recombinant mouse p38 protein.|Specificity: Reacts specifically with p38-a-MAP kinase and does not crossreact with other MAP kinases. The immunoreactive epitope has not yet been identified. Reactivity was confirmed by Western Blot using lysates derived from the following cell lines: J774A.1 (mouse macrophage), BHK hamster kidney), CHO (hamster ovary), COS (monkey kidney), Jurkat, PC-12, NIH 3T3, C2C12 (mouse myoblast) and L929. Species Crossreactivity: Human, mouse, rat, hamster and monkey.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Full-length recombinant mouse p38 protein.
Form
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
Purity
Purified by Protein A affinity chromatography.
Specificity
Reacts specifically with p38-a-MAP kinase and does not crossreact with other MAP kinases. The immunoreactive epitope has not yet been identified. Reactivity was confirmed by Western Blot using lysates derived from the following cell lines: J774A.1 (mouse macrophage), BHK hamster kidney), CHO (hamster ovary), COS (monkey kidney), Jurkat, PC-12, NIH 3T3, C2C12 (mouse myoblast) and L929. Species Crossreactivity: Human, mouse, rat, hamster and monkey.
References
1. Davis, R.J., Trends in Biochem. Sci. 19: 470-473 (1994). 2. Han, J., et al., Science 265: 808-811 (1994). 3. Lee, J.C., et al., Nature 372: 739-746 (1994). 4. Raingeaud, J., et al., J. Biol. Chem. 270: 7420-7426 (1995). 5. Derijard, B., et al., Science 267: 682 (1995). 6. Raingeaud, J., et al., Mol. Cell. Biol. 16: 1247-1255 (1996). 7. Freshney, N.W., et al., Cell 78: 1039-1049 (1994). 8. Rouse, J.P., Cell 78: 1027-1037 (1994). 9. Zhang, S.J., et al., J. Biol. Chem. 270: 23,934-23,936 (1995). 10. McLaughlin, M.M., et al., J. Biol. Chem. 271: 8488-8492 (1996). 11. Wang, X., et al., Science 272: 1347-1349 (1996). 12. Xia, Z., et al., Science 270: 1326-1331 (1995).