MAPK7 Positive Control

Formulation:	Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Dilution Range:	WB: 1:500
Storage Instruction:	Store at-20°C for long term storage. Avoid freeze-thaw cycles.

Gene Symbol:	MAPK7
Gene ID:	5598
Uniprot ID:	MK07_HUMAN
Specificity:	This is positive control is recommended for use in combination with MAPK7 antibody STJ501713.

Tissue Specificity	Expressed in many adult tissues. Abundant in heart, placenta, lung, kidney and skeletal muscle. Not detectable in liver.
Post Translational Modifications	Dually phosphorylated on Thr-219 and Tyr-221, which activates the enzyme. Autophosphorylated in vitro on threonine and tyrosine residues when the C-terminal part of the kinase, which could have a regulatory role, is absent.
Function	Plays a role in various cellular processes such as proliferation, differentiation and cell survival. The upstream activator of MAPK7 is the MAPK kinase MAP2K5. Upon activation, it translocates to the nucleus and phosphorylates various downstream targets including MEF2C. EGF activates MAPK7 through a Ras-independent and MAP2K5-dependent pathway. May have a role in muscle cell differentiation. May be important for endothelial function and maintenance of blood vessel integrity. MAP2K5 and MAPK7 interact specifically with one another and not with MEK1/ERK1 or MEK2/ERK2 pathways. Phosphorylates SGK1 at Ser-78 and this is required for growth factor-induced cell cycle progression. Involved in the regulation of p53/TP53 by disrupting the PML-MDM2 interaction.
Peptide Name	Mitogen-Activated Protein Kinase 7 Map Kinase 7 Mapk 7 Big Map Kinase 1 Bmk-1 Extracellular Signal-Regulated Kinase 5 Erk-5
Database Links	Reactome: R-HSA-198753 Reactome: R-HSA-198765 Reactome: R-HSA-202670 Reactome: R-HSA-2559582 Reactome: R-HSA-881907 Reactome: R-HSA-8853659
Cellular Localisation	Cytoplasm Nucleus Pml Body Translocates To The Nucleus Upon Activation
Alternative Peptide Names	Mitogen-Activated Protein Kinase 7 protein Map Kinase 7 protein Mapk 7 protein Big Map Kinase 1 protein Bmk-1 protein Extracellular Signal-Regulated Kinase 5 protein Erk-5 protein MAPK7 protein BMK1 protein ERK5 protein PRKM7 protein

Information sourced from Uniprot.org

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