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> MB Polyclonal Antibody

MB Polyclonal Antibody

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Cat# E-AB-40504-200

Size : 200uL

Brand : Elabscience

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Phone : +1 850 650 7790

Verified Samples Verified Samples in WB: Zebrafish, MCF7
Dilution WB 1:1000-5000
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat,  Zebrafish
Applications WB
Clonality Polyclonal
Immunogen Recombinant human MB protein expressed by E.coli
Abbre MB
Synonyms MYG,   Myoglobin,   PVALB,  MGC13548
Swissprot
Calculated MW 16 kDa
Observed MW 16 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization Cytosol, Extracellular region or secreted, extracellular exosome.
Concentration 1 mg/mL
Buffer PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4
Purification Method Antigen Affinity Purification
Research Areas Cancer,  Cardiovascular,  Metabolism
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Myoglobin (symbol Mb or MB) is an iron- and oxygen-binding protein found in the muscle tissue of vertebrates in general and in almost all mammals. Myoglobin is released from damaged muscle tissue such as heart, making it a sensitive marker for muscle inju
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Other Clones

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Unconjugated

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