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> Methylene Blue [61-73-4]

Methylene Blue [61-73-4]

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Cat# HY-14536-500mg

Size : 500mg

Brand : MedChemExpress

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Methylene blue (Basic Blue 9) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue is a Tau aggregation inhibitor. Methylene blue reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation.

For research use only. We do not sell to patients.

Methylene Blue Chemical Structure

Methylene Blue Chemical Structure

CAS No. : 61-73-4

This product is a controlled substance and not for sale in your territory.

Based on 13 publication(s) in Google Scholar

Other Forms of Methylene Blue:

  • Methylene blue trihydrate In-stock
  • Methylene blue hydrate In-stock
  • Methylene blue (purity≥70%) Get quote

View All Monoamine Oxidase Isoform Specific Products:

View All Isoforms
MAO-A MAO-B

View All NO Synthase Isoform Specific Products:

View All Isoforms
nNOS iNOS eNOS
Description

Methylene blue (Basic Blue 9) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue is a Tau aggregation inhibitor. Methylene blue reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation[1][2][3].

IC50 & Target

Guanylyl cyclase (sGC)[1].
Monoamine oxidase A (MAO-A)[1].
NO synthase (NOS)[1]
In Vitro

Methylene blue (Basic Blue 9) (4.5 μM; BV2 microglia) alters the immune profile of LPS-activated BV2 microglia and decreases the level of CD14, IL-1β, TNF-α, and CCL2 mRNA[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Methylene Blue Related Antibodies
In Vivo

Methylene blue (Basic Blue 9) (50 and 100 mg/kg; i.p.; once, for 25 min; male NMRI mice) reduces absent prepulse inhibition[1].
Methylene blue (Basic Blue 9) (20 and 40 mg/kg; p.o.; daily, for 6 months; CaMKIIα-tTA transactivator mice) preserves cognition in mice expressing full-length pro-aggregant human Tau[2].
Methylene blue (Basic Blue 9) (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces TBI-induced edema and neuroinflammation and reduces acute depression-like behavior[3].
Methylene blue (Basic Blue 9) (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces the percentage of inflammatory factor[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male NMRI mice[1]
Dosage: 50 and 100 mg/kg
Administration: Intraperitoneal injection; once, for 25 minutes
Result: Reduced the prepulse inhibition and reduced the increase in locomotor activity caused by phencyclidine (PCP).
Animal Model: CaMKIIα-tTA transactivator mice[2]
Dosage: 20 and 40 mg/kg
Administration: Oral administration; daily, for 6 months
Result: Inhibited Tau aggregation in CaMKIIα-tTA transactivator mice.
Animal Model: TBI-treated male BALB/c mice[3]
Dosage: 2 mg/kg
Administration: Intravenous injection; once, for 1 day
Result: Decreased the level of CD14, IL-1β, TNF-α, and CCL2 mRNA.
Animal Model: TBI-treated male BALB/c mice[3]
Dosage: 2 mg/kg
Administration: Intravenous injection; once, for 1 day
Result: Reduced the percentage of myeloid (CD11b+/GR1+) cells, reduced IL-1β and enhanced IL-10 expression in microglia.
Clinical Trial
Molecular Weight

319.85

Formula

C16H18ClN3S
CAS No.

61-73-4
Appearance

Solid

Color

Reddish brown to brown

SMILES

CN(C)C1=CC=C2C(SC(C(C=C/3)=N2)=CC3=[N+](C)/C)=C1.[Cl-]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Store at room temperature, keep dry and cool
Solvent & Solubility
In Vitro: 

H2O : 50 mg/mL (156.32 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1265 mL 15.6323 mL 31.2647 mL
5 mM 0.6253 mL 3.1265 mL 6.2529 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: 98.08%

COA (246 KB) HNMR (246 KB) LCMS (263 KB)

Handling Instructions (2659 KB)
Dyeing Example
  • Description: Methylene Blue can be used for cellular staining in colony formation assay.
    Method: For cell staining.
    1. Fix colonies with methanol/acetone (1:1).
    2. Stain cells with 0.5% Methylene Blue.
  • Description: Methylene Blue can be used for cellular staining in colony formation assay.
    Method: For cell staining.
    1. Fix colonies with methanol/acetone (1:1).
    2. Stain cells with 0.5% Methylene Blue.
    3. Use a Nikon TE200 microscope for colonies counting.
References

  • [1]. Klamer D, et, al. Phencyclidine-induced behaviour in mice prevented by methylene blue. Basic Clin Pharmacol Toxicol. 2004 Feb;94(2):65-72.  [Content Brief]

    [2]. Hochgräfe K, et, al. Preventive methylene blue treatment preserves cognition in mice expressing full-length pro-aggregant human Tau. Acta Neuropathol Commun. 2015 May 10;3:25.  [Content Brief]

    [3]. Fenn AM, et, al. Methylene blue attenuates traumatic brain injury-associated neuroinflammation and acute depressive-like behavior in mice. J Neurotrauma. 2015 Jan 15;32(2):127-38.  [Content Brief]

  • [1]. Klamer D, et, al. Phencyclidine-induced behaviour in mice prevented by methylene blue. Basic Clin Pharmacol Toxicol. 2004 Feb;94(2):65-72.

    [2]. Hochgräfe K, et, al. Preventive methylene blue treatment preserves cognition in mice expressing full-length pro-aggregant human Tau. Acta Neuropathol Commun. 2015 May 10;3:25.

    [3]. Fenn AM, et, al. Methylene blue attenuates traumatic brain injury-associated neuroinflammation and acute depressive-like behavior in mice. J Neurotrauma. 2015 Jan 15;32(2):127-38.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 3.1265 mL 15.6323 mL 31.2647 mL 78.1616 mL
5 mM 0.6253 mL 3.1265 mL 6.2529 mL 15.6323 mL
10 mM 0.3126 mL 1.5632 mL 3.1265 mL 7.8162 mL
15 mM 0.2084 mL 1.0422 mL 2.0843 mL 5.2108 mL
20 mM 0.1563 mL 0.7816 mL 1.5632 mL 3.9081 mL
25 mM 0.1251 mL 0.6253 mL 1.2506 mL 3.1265 mL
30 mM 0.1042 mL 0.5211 mL 1.0422 mL 2.6054 mL
40 mM 0.0782 mL 0.3908 mL 0.7816 mL 1.9540 mL
50 mM 0.0625 mL 0.3126 mL 0.6253 mL 1.5632 mL
60 mM 0.0521 mL 0.2605 mL 0.5211 mL 1.3027 mL
80 mM 0.0391 mL 0.1954 mL 0.3908 mL 0.9770 mL
100 mM 0.0313 mL 0.1563 mL 0.3126 mL 0.7816 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Methylene Blue Related Classifications

  • Fluorescent Dye
  • Cell Staining Analysis
  • Cell Function Analysiss
  • Neuroscience Analysis dyes
  • Neurological Disease Infection Cancer
  • Cancer Targeted Therapy Cancer Immunotherapy Cancer Metabolism and Metastasis
  • GPCR/G Protein Neuronal Signaling Immunology/Inflammation Cell Cycle/DNA Damage Cytoskeleton
  • Guanylate Cyclase Monoamine Oxidase NO Synthase Microtubule/Tubulin
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methylene Blue61-73-4Basic Blue 9 CI-52015MethylthioniniumBasic Blue9Basic Blue-9CI52015CI 52015CI-52015Guanylate CyclaseMonoamine OxidaseNO SynthaseMicrotubule/TubulinMAONitric oxide synthasesNOSvasopressordyeantinociceptionantimalarialantidepressantanxiolyticmethemoglobinemiascGMPInhibitorinhibitorinhibit

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