MitoTracker Deep Red FM [873315-86-7]
Cat# HY-D1783-50ug
Size : 50ug
Brand : MedChemExpress
MitoTracker Deep Red FM fluorescent dye that selectively accumulates in the mitochondrial matrix. MitoTracker Deep Red FM covalently binds mitochondrial proteins by reacting with free mercaptan of cysteine residues, allowing staining of mitochondrial membrane potential independent of membrane potential. Excitation/emission wavelength 644/665 nm. Storage: Keep away from light.
For research use only. We do not sell to patients.
MitoTracker Deep Red FM Chemical Structure
CAS No. : 873315-86-7
This product is a controlled substance and not for sale in your territory.
Based on 1 publication(s) in Google Scholar
Description |
MitoTracker Deep Red FM fluorescent dye that selectively accumulates in the mitochondrial matrix. MitoTracker Deep Red FM covalently binds mitochondrial proteins by reacting with free mercaptan of cysteine residues, allowing staining of mitochondrial membrane potential independent of membrane potential. Excitation/emission wavelength 644/665 nm[1]. Storage: Keep away from light. |
||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
In Vitro |
General Protocol MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. |
||||||||||||
Molecular Weight |
543.57 |
||||||||||||
Formula |
C34H36Cl2N2 |
||||||||||||
CAS No. |
873315-86-7 |
||||||||||||
Appearance |
Solid |
||||||||||||
Color |
Blue to dark blue |
||||||||||||
SMILES |
ClCC1=CC=C(CN2C3=C(C(C)(C)/C2=C/C=C/C=C/C4=[N+](C)C5=C(C4(C)C)C=CC=C5)C=CC=C3)C=C1.[Cl-] |
||||||||||||
Shipping | Room temperature in continental US; may vary elsewhere. |
||||||||||||
Storage |
-20°C, protect from light, stored under nitrogen *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen) |
||||||||||||
Solvent & Solubility |
In Vitro:
DMSO : 125 mg/mL (229.96 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO) Preparing
Stock Solutions
View the Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol) Concentration (start) × Volume (start) = Concentration (final) × Volume (final) This equation is commonly abbreviated as: C1V1 = C2V2 In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
Dosage mg/kgAnimal weight Dosing volume Number of animals Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration:
mg/mL
|
||||||||||||
Purity & Documentation |
Purity: ≥99.0% |
||||||||||||
References |
|
Complete Stock Solution Preparation Table
*
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
---|
DMSO | 1 mM | 1.8397 mL | 9.1984 mL | 18.3969 mL | 45.9922 mL |
5 mM | 0.3679 mL | 1.8397 mL | 3.6794 mL | 9.1984 mL | |
10 mM | 0.1840 mL | 0.9198 mL | 1.8397 mL | 4.5992 mL | |
15 mM | 0.1226 mL | 0.6132 mL | 1.2265 mL | 3.0661 mL | |
20 mM | 0.0920 mL | 0.4599 mL | 0.9198 mL | 2.2996 mL | |
25 mM | 0.0736 mL | 0.3679 mL | 0.7359 mL | 1.8397 mL | |
30 mM | 0.0613 mL | 0.3066 mL | 0.6132 mL | 1.5331 mL | |
40 mM | 0.0460 mL | 0.2300 mL | 0.4599 mL | 1.1498 mL | |
50 mM | 0.0368 mL | 0.1840 mL | 0.3679 mL | 0.9198 mL | |
60 mM | 0.0307 mL | 0.1533 mL | 0.3066 mL | 0.7665 mL | |
80 mM | 0.0230 mL | 0.1150 mL | 0.2300 mL | 0.5749 mL | |
100 mM | 0.0184 mL | 0.0920 mL | 0.1840 mL | 0.4599 mL |
MitoTracker Deep Red FM Related Classifications
- Others
- Fluorescent Dye
-
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Keywords:
MitoTracker Deep Red FM873315-86-7Fluorescent Dyered fluorescent dyemitochondriafixationpermeationInhibitorinhibitorinhibit