Label your antibody with one of our CF® Dyes specialized for STORM super-resolution microscopy. Labeling takes just 30 minutes without a purification step, and yields an antibody conjugate with a low degree of labeling optimal for STORM.
Technology
Antibody & Protein Labeling Kits
CF® Dyes
CF® Dyes for Multi-Color Super-Resolution Microscopy
Antibody & Protein Labeling Kits
Tech Tips
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host
The Full Spectrum Blog
Blog: An Interview with STORM expert Ke Xu on Super-Resolution Microscopy
Blog: Pushing Biology Forward with Super Resolution
Highlighted Citations
UC Berkeley and Biotium identify dye modifications for superior fluorescence photoswitching in STORM
This product is available by special order only.
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Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits allow you to label 50 ug of your antibody with one of Biotium’s STORM CF® Dyes to produce an antibody conjugate with a low 1-2.5 DOL (degree of labeling) optimal for super-resolution STORM (Stochastic Optical Reconstruction Microscopy). Labeling takes just 30 minutes, with minimal hands-on time and no purification.
Features
Label 50 ug antibody
Choice of 8 CF® Dye colors ideal for STORM
CF®583R and CF®597R are novel green-excited STORM dyes
Labeling optimized to provide low 1-2.5 DOL
Less than 30 seconds of hands-on time
30 minutes total reaction time
No purification, 100% recovery
Kit components
Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
10X Mix-n-Stain™ Reaction Buffer
Lyophilized Reactive STORM CF® Dye
Mix-n-Stain™ Storage Buffer
Superior CF® Dyes for STORM Imaging
These kits feature a choice of 8 CF® Dyes developed with superior brightness and unique photoswitching properties that are ideal for super-resolution imaging by STORM. In addition, these specialized Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits are designed to offer a low degree of labeling (DOL, or dye molecules per antibody molecule) of 1-2.5, which is reported to be optimal for STORM (1). Learn more about CF® Dyes for STORM or our other fluorescent reagents validated for super-resolution microscopy.
Discover More with Green-Excitable CF® Dyes Developed for Multi-Color STORM
High-quality STORM imaging relies heavily on bright fluorescent dyes with excellent photoswitching properties. While there are several commonly used red-excited dyes suitable for STORM imaging such as CF®647, CF®660C and CF®680, generating comparable dyes for other excitation sources to produce quality multi-color STORM images remains a challenge. Biotium scientists have met this challenge by developing novel chemical modifications for green-excited fluorescent dyes to offer optimal performance for STORM super-resolution imaging . This includes CF®583R and CF®597R which were developed specifically for STORM imaging and display exceptional performance for multi-color STORM (2).
See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye. Select the links in the Dye Features column to learn more about each CF® Dye and potential applications.
Other Labeling Kits
We now offer Mix-n-Stain™ Maxi Antibody Labeling Kits for labeling 1 mg of antibody with CF® Dyes, and Mix-n-Stain™ Nanobody Labeling Kits. Mix-n-Stain™ kits also are available for labeling antibodies biotin, DNP, or DIG. See our full selection of Mix-n-Stain™ Antibody Labeling Kits.