Reagents and instruments for immunology, cell biology and molecular biology.
 
> MOUSE IgG3 antibody (ATTO 425)

MOUSE IgG3 antibody (ATTO 425)

Print Print
Please log in

Cat# orb347442-500ug

Size : 500ug

Brand : Biorbyt

Contact local distributor :


Phone : +1 850 650 7790

    • Home
    • MOUSE IgG3 antibody (ATTO 425) [Out of stock]

    MOUSE IgG3 antibody (ATTO 425) [Out of stock]

    Catalog Number: orb347442

    Catalog Numberorb347442
    CategoryAntibodies
    DescriptionMOUSE IgG3 antibody (ATTO 425)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsDOT, FC, FLISA, IF, WB
    ReactivityMouse
    IsotypeIgG
    ImmunogenMouse IgG3 heavy chain
    Concentration1.0 mg/mL
    Dilution rangeFLISA: >1:20,000, FC: 1:500 - 1:2,500, IF: >1:5,000, WB: 1:4,000 - 1:20,000
    Form/AppearanceLyophilized
    PurityAnti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG3. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.
    ConjugationATTO-425
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesgoat anti-MOUSE IgG3 Antibody ATTO425 Conjugation,
    NoteFor research use only
    Application notesMouse IgG3 secondary antibody is available in a variety of formats. Anti-Mouse IgG3 ATTO 425 Antibody has been tested by dot blot. ATTO 425 conjugations are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
    Expiration Date12 months from date of receipt.
    MOUSE IgG3 antibody (ATTO 425) [Out of stock]

    ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.

    MOUSE IgG3 antibody (ATTO 425) [Out of stock]

    ATTO 425 conjugated anti-Mouse IgG was used to demonstrate 2 color STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 µg/mL final concentration of anti-a-tubulin and detected with 0.2 µg/mL ATTO 425 conjugated anti-MOUSE IgG [GOAT] (orb347443) secondary antibody (colored RED). Also shown in this 2-color STED image is Biorbyt's Anti-HDAC-1 [RABBIT] (p/n orb345508) detected with DyLight™488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody (colored GREEN).