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Mouse Monoclonal Anti-Blood Group Antigen A (CD173) [Clone HE-14]
Cat# NB-36-00245-P1
Size : 100ug
Brand : Neo Biotech
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Printable Datasheet
Blood Group Antigen A (CD173) Antibody [HE-14]
Catalog Number Formulation Size Price
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Applications & Dilutions
Applications Tested Dillution Protocol
Immunofluorescence (IF)
1-3ug/ml
Summary
This antibody recognizes human blood group A (monofucosyl and difucosyl A antigens with chain types 1, 2, 3, 4, 5, 6) and Forssmann antigen. It is also reactive with the immuno-dominant A trisaccharide. Blood group antigen expression in human colon cancer was studied by means of two monoclonal antibodies of broad anti-A (HE-14) and anti-type 3 and type 4 chain-based A and H (HE-10) specificity. These antigens were proved to re-appear in tumors of the distal colon, the HE-10 antibody reacting more frequently (9 out of 12 samples) than HE-14 (5 out of 12 samples) and frequently with supra-nuclear staining of the cytoplasm probably in those places of the Golgi apparatus where carbohydrate antigens are synthesized. This staining pattern is characteristic of HE-10 in normal colonic mucosa as well. With HE-14, staining was often absent in less differentiated tumors, while HE-10 did react in such tumors. In some cases, these two antibodies gave different staining patterns in parallel sections from the same tissue sample, primarily at the cellular level. Three out of 12 cases showed blood group antigen expression in the mucosa of the distal colon adjacent to the tumor only when HE-10 Ab was used.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgM / Kappa
Cellular Localization
Golgi apparatus, Golgi stack membrane, Secreted
Gene Name
ABO
Positive Control
KG1 cells or human colorectal carcinoma tissues.
Immunogen
Mixture of erythrocytes of blood group A1and glycoprotein fraction isolated from the saliva of secretors with blood group A
Alternate Names
Histo-blood group ABO system transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Histo-blood group A transferase, Histo-blood group B transferase, NAGAT, A transferase; ABO; B transferase; CD173; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; Histo-blood group A transferase; Histo-blood group B transferase; NAGAT
Functions
- This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity.
PubMed Links
- https://pubmed.ncbi.nlm.nih.gov/2104828/
- https://pubmed.ncbi.nlm.nih.gov/2333095/
- https://pubmed.ncbi.nlm.nih.gov/7598760/
- https://pubmed.ncbi.nlm.nih.gov/7779106/
- https://pubmed.ncbi.nlm.nih.gov/16533287/
- https://pubmed.ncbi.nlm.nih.gov/17764507/
- https://pubmed.ncbi.nlm.nih.gov/18513251/
- https://pubmed.ncbi.nlm.nih.gov/15489334/
- https://pubmed.ncbi.nlm.nih.gov/12829588/
- https://pubmed.ncbi.nlm.nih.gov/8839869/
- https://pubmed.ncbi.nlm.nih.gov/9800297/
- https://pubmed.ncbi.nlm.nih.gov/15104652/
- https://pubmed.ncbi.nlm.nih.gov/1449469/
- https://pubmed.ncbi.nlm.nih.gov/2121736/
- https://pubmed.ncbi.nlm.nih.gov/10462501/
- https://pubmed.ncbi.nlm.nih.gov/12198488/
- https://pubmed.ncbi.nlm.nih.gov/12529355/
- https://pubmed.ncbi.nlm.nih.gov/12972418/
- https://pubmed.ncbi.nlm.nih.gov/15475562/
- https://pubmed.ncbi.nlm.nih.gov/16326711/
- https://pubmed.ncbi.nlm.nih.gov/17259183/
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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