PARTNERSHIP OPPORTUNITIES
Mouse Monoclonal Anti-Plakophilin-1 (PKP1) [Clone 10B2]
Cat# NB-36-00614-P1
Size : 100ug
Brand : Neo Biotech
Contact local distributor :
Phone : +1 850 650 7790
Free Shipping in the U.S. for orders over $1000. Shop Now>>
Printable Datasheet
Plakophilin-1 (PKP1) Antibody [10B2]
Catalog Number Formulation Size Price
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Western Blot of U87, HeLa and A431 cell lysates using Plakophilin-1 Mouse Monoclonal Antibody (10B2)
SDS-PAGE Analysis of Purified CD56 Monoclonal Antibody (NCAM1/784). Confirmation of Integrity and Purity of Antibody
Applications & Dilutions
Applications Tested Dillution Protocol
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Western Blot (WB)
2-4ug/ml
Western Blot Protocol
Summary
Recognizes a protein of 75kDa, identified as Plakophilin-1. Its epitope maps between aa 1-27 human Plakophilin-1. Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. Plakophilins 1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). Plakophilin 2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.
Product Properties & Targets
Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Western Blot
Species Reactivity
Human, Mouse
Research Areas
Apoptosis, Autophagy, Cardiovascular, Developmental Biology, Immunology, Nuclear Marker
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell junction, Desmosome, Nucleus
Gene Name
PKP1
Positive Control
HeLa and A431 cells., U87
Immunogen
N-terminus of human Plakophilin-1 protein
Alternate Names
Plakophilin-1, Band 6 protein, B6P; Band 6 protein; PKP1; Plakophilin-1 (ectodermal dysplasia/skin fragility syndrome)
Functions
- Seems to play a role in junctional plaques. Contributes to epidermal morphogenesis.
Key References
- Hadiji-Abbes, N. et al. 2014. Negative control glucose dependent mediated by the PreS2 region on the translation efficiency of the reporter Sh-bleomycin gene in Saccharomyces cerevisiae. FEMS Yeast Res. 14: 357-63.
PubMed Links
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
There are no reviews yet.