NCR1 Mouse Monoclonal Antibody [Clone ID: AKS1]

Specifications

Product Data
Clone Name	AKS1
Applications	FC, FN, IF, WB
Recommended Dilution	Western Blot. Immunofluorescence. Functional Assays. Flow Cytometry: Use 10 µl of 1/5-1/200 diluted antibody to label 10e6 cells in 100 µl.
Reactivities	Bovine
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Fusion protein consisting of the extracellular region of Bovine CD335.
Specificity	This Low Endotoxin antibody is specific for Bovine CD335, also known as NKp46 and Natural cytotoxicity triggering receptor 1. Clone AKS1 is reported to activate lysis of FcgammaR-expressing cell line P815, by IL-2 activated NKp46+ cells (2).
Formulation	PBS, pH 7.4 containing no preservatives. State: Low Endotoxin State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage	Store the antibody undiluted at -20°C. Should this product contain a precipitate we recommend microcentrifugation before use. Avoid repeated freezing and thawing
Stability	Shelf life: one year from despatch.
Database Link	Entrez Gene 369024 Bovine UniProt ID Q863H2
Background	CD335 is a type I transmembrane protein, with two extracellular C2-type immunoglobulin-like domains, which functions as an activating receptor. CD335 is expressed by human natural killer cells (4). The bovine homologue is expressed on bovine NK cells (2) and no expression of CD335 has been detected on B cells, T cells, monocytes or granulocytes.
Synonyms	NCR1, LY94, NKp46
Note	Endotoxin Level: < 0.01EU/ug.
Reference Data