Nitric Oxide Synthase BioAssay™ Kit (NOS)

Sample Type:|Cell culture||Excitation/Emission:|488nm/515nm||Method of Analysis:|Flow Cytometry, Fluorescence Microscope, Fluorescence Plate Reader||Intended Use:|Nitric Oxide Synthase Assay provides a good screening option for assessing the potency of nitrosative stress inhibitor and activator reagents, and will help to determine how oxidative and nitrosative stress modulates varied intracellular pathways. This BioAssay™ kit assesses the overall intracellular levels of free nitric oxide and NOS using a Diaminofluorescein-2 Diacetate (DAF-2DA) dye. Results can be analyzed using a flow cytometer, fluorescence plate reader, or fluorescence microscope.||Not for sale in Japan.||Test Principle:|Nitric oxide synthases are a family of enzymes capable of catalyzing the production of nitric oxide (NO). NO is an important molecule that is involved in regulating a variety of cellular processes such as angiogenesis, peristalsis, and the immune response to invading pathogens. Reactive nitrogen species are a family of antimicrobial molecules derived from NO and superoxide through the enzymatic activities of nitric oxide synthase (NOS).||Nitric Oxide Synthase BioAssay™ provides a good screening option for assessing the potency of nitrosative stress inhibitor and activator reagents, and will help to determine how oxidative and nitrosative stress modulates varied intracellular pathways. This BioAssay™ kit assesses the overall intracellular levels of free nitric oxide and NOS using a Diaminofluorescein-2 Diacetate (DAF-2DA) dye. Results can be analyzed using a flow cytometry, fluorescence plate reader, or fluorescence microscope.||Not for sale in Japan.||Kit Components:|50-100 tests:|Diaminofluorescein-2 Diacetate (DAF-2DA)|Hoechst 33342|10X BioAssay™ Buffer, 1x60mL||Storage and Stability:|Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.