NP Rabbit Polyclonal Antibody
Cat# AP21354AF-N
Size : 10mg
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| Product Data | |
| Application | ELISA, ID, IF, IP, R, WB |
|---|---|
| Application | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of choice. Working Dilutions: Non-precipitating antibody-binding techniques: 1/1,000-1/20,000. |
| Reactivity | Bovine |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Nucleoside phosphorylase isolated and purified from calf spleen. Freund’s complete adjuvant is used in the first step of the immunization procedure. |
| Specificity | Nucleoside Phosphorylase from Calf spleen. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. |
| Buffer | PBS, pH 7.2 without preservatives and foreign proteins State: Azide Free State: Lyophilized hyperimmune IgG fraction |
| Reconstitution Method | Restore by adding 1.0 ml of sterile distilled water |
| Concentration | lot specific |
| Purification | Ammonium Sulphate Precipitation and Ion Exchange Chromatography |
| Conjugation | Unconjugated |
| Storage | Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation. |
| Stability | Shelf life: one year from despatch. |
| Database Link | |
| Background | This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. This enzyme, together with adenosine deaminase (ADA) serves a key role in purine catabolism. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2. |
| Synonyms | PNP, PNP1, Inosine Phosphorylase |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.