PCNA antibody [PC10] (FITC)
Cat# GTX75144-50ug
Size : 50ug
Brand : GeneTex
Contact local distributor :
Phone : +1 850 650 7790
![Anti-PCNA antibody [PC10] (FITC) used in Flow cytometry (FACS). GTX75144](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-PCNA antibody [PC10] (FITC) used in Flow cytometry (FACS). GTX75144")
Host | Mouse |
|---|---|
Clonality | Monoclonal |
Clone Name | PC10 |
Isotype | IgG2a |
Application | FACS, IHC |
Reactivity | Human, Mouse, Rat, Rabbit, Sheep, Cat, Dog, Hamster, Chicken, Atlantic salmon, Cynomolgus monkey, Ferret, Horse, Rhesus Monkey, Xenopus |
| Package | 50 μg |
Datasheet File View product citations for antibody GTX75144 on CiteAb
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application | Recommended Dilution |
|---|---|
| FACS | Neat-1/10 |
| IHC | Assay dependent |
| Note : FACSMembrane permeabilization is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl. | |
Not tested in other applications.
Calculated MW
29 kDa. ( Note )
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. Protect from light.
Concentration
0.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Rat
Immunogen
Rat PCNA made in the protein A expression vector pR1T2T
Purification
Protein G purified
From tissue culture supernatant
From tissue culture supernatant
Conjugation
Fluorescein isothiocyanate (FITC) Wavelength
RRID
AB_376619
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
proliferating cell nuclear antigen , PCNAR , Pcna/cyclin
Cellular Localization
Nucleus
Background
cell cycle protein that may contribute to the repair of DNA damage and to cell proliferation caused by exposure to pulmonary toxicants; may influence cell survival in ischaemically compromised tissue [RGD, Feb 2006]
Database
DATA IMAGES
| | GTX75144 FACS ImageFACS analysis of KM-H2 cells using GTX75144 PCNA antibody [PC10] (FITC). |
Clone Reference
Yeom M et al. Chem Res Toxicol 2020; 33 (8):2120-2129 Three Human Pol ι Variants with Impaired Polymerase Activity Fail to Rescue H2O2 Sensitivity in POLI-Deficient Cells.
Bryche B et al. Neural Regen Res 2020; 15 (2):352-360 Endothelin increases the proliferation of rat olfactory mucosa cells.
Ketteler J et al. J Clin Med 2019; 8 (3) Progression-Related Loss of Stromal Caveolin 1 Levels Mediates Radiation Resistance in Prostate Carcinoma via the Apoptosis Inhibitor TRIAP1.
Bryche B et al. Brain Behav Immun 2019; (Epub) IL-17c is involved in olfactory mucosa responses to Poly(I:C) mimicking virus presence.
?ensoy E et al. Turk J Gastroenterol 2019; 30 (10):917-924 Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods.
Submit a Reference REVIEW
Application Reference
Tomas J et al. FASEB J 2013; 27 (2):645-55 Primocolonization is associated with colonic epithelial maturation during conventionalization.
Application : IHC
Reactivity : Rat
Clone Reference
Yeom M et al. Chem Res Toxicol 2020; 33 (8):2120-2129 Three Human Pol ι Variants with Impaired Polymerase Activity Fail to Rescue H2O2 Sensitivity in POLI-Deficient Cells.
Bryche B et al. Neural Regen Res 2020; 15 (2):352-360 Endothelin increases the proliferation of rat olfactory mucosa cells.
Ketteler J et al. J Clin Med 2019; 8 (3) Progression-Related Loss of Stromal Caveolin 1 Levels Mediates Radiation Resistance in Prostate Carcinoma via the Apoptosis Inhibitor TRIAP1.
Bryche B et al. Brain Behav Immun 2019; (Epub) IL-17c is involved in olfactory mucosa responses to Poly(I:C) mimicking virus presence.
?ensoy E et al. Turk J Gastroenterol 2019; 30 (10):917-924 Determination of the changes on the small intestine of pregnant mice by histological, enzyme histochemical, and immunohistochemical methods.
Chauvigne F et al. PLoS One 2017; 12 (3):e0174387 toward developing recombinant *-based hormone therapies for increasing fertility in the flatfish senegalese sole.
Chauvigne F et al. Comp Biochem Physiol A Mol Integr Physiol 2017; 206:36-46 Dual estrogenic regulation of the nuclear progestin receptor and spermatogonial renewal during gilthead seabream (Sparus aurata) spermatogenesis.
Riviere S et al. Sci Rep 2016; 6:34011 High Fructose Diet inducing diabetes rapidly impacts olfactory epithelium and behavior in mice.
Francois A et al. Sci Rep 2016; 6:24687 Olfactory epithelium changes in germfree mice.
Ozaydin T et al. Microsc Res Tech 2016; Immunohistochemical distribution of heat shock protein 70 and proliferating cell nuclear antigen in mouse placenta at different gestational stages.
Gu LH et al. Cell Mol Immunol 2015; 12 (4):444-55 Immunogenicity of allogeneic mesenchymal stem cells transplanted via different routes in diabetic rats.
Boj M et al. PLoS One 2015; 10 (11):e0142512 *-activated androgen-dependent and independent pathways regulate aquaporin expression during teleost (sparus aurata) spermatogenesis.
Aurelien Raynaud et al. Behavioural Brain Research 2015; 15 (291):36-45 Chronic variable stress exposure in male Wistar rats affects the first step of olfactory detection
Liu Y et al. Cytotechnology 2014; Lupeol induces apoptosis and inhibits invasion in gallbladder carcinoma GBC-SD cells by suppression of EGFR/MMP-9 signaling pathway.
Turpin W et al. PLoS One 2013; 8 (4):e57711 Behavior of lactobacilli isolated from fermented slurry (ben-saalga) in gnotobiotic rats.
Gultekin FA et al. J Invest Surg 2013; 26 (5):242-52 Effects of ozone oxidative preconditioning on liver regeneration after partial hepatectomy in rats.
Rul F et al. J Biol Chem 2011; 286 (12):10288-96 Impact of the metabolic activity of Streptococcus thermophilus on the colon epithelium of gnotobiotic rats.
Cherbuy C et al. Am J Physiol Gastrointest Liver Physiol 2010; 299 (2):G348-57 Microbiota matures colonic epithelium through a coordinated induction of cell cycle-related proteins in gnotobiotic rat.
Guo Z et al. Nat Chem Biol 2010; 6 (10):766-73 Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding.
Ji RC et al. Microvasc Res 2010; 80 (2):195-201 Multiple expressions of lymphatic markers and morphological evolution of newly formed lymphatics in lymphangioma and lymph node lymphangiogenesis.
Kumar R et al. Clin Cancer Res 2009; 15 (12):4123-30 extranuclear coactivator signaling confers insensitivity to *.
Yasemin O et al. Journal of Animal and Veterinary Advances. 2009;:8(9):1694-701. Comparative Skin Histology of the White New Zealand and Angora Rabbits: Histometrical and Immunohistochemical Evaluations.
Application : IHC
Krasna L et al. Breast Cancer Res Treat 2002; 71 (3):219-35 Large expansion of morphologically heterogeneous mammary epithelial cells, including the luminal phenotype, from human breast tumours.
| SDS | |
|---|---|
| PBS.pdf | |
| Sodium Azide.pdf | |