pEASY®-Blunt Simple Cloning Kit
Cat# CB111-01
Size : 20rxns
Brand : TransGen Biotech
Contact local distributor :
Phone : +1 850 650 7790
ManualpEASY®-Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products.
•5 minutes fast ligation of Pfu-amplified PCR products.
•Kanamycin and Ampicillin resistance genes for selection.
•Easy blue/white selection.
•SR primer and M13 forward primer for sequencing.
•T7 promoter for in vitro transcription.
•Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (>109 cfu/μg pUC19 DNA) and fast growing.
Storage
Trans1-T1 Phage Resistant Chemically Competent Cell at -70°C for six months; others at -20°C for nine months
Shipping
Dry ice (-70℃)
| Component | CB111-01 (20 rxns) | CB111-02 (60 rxns) |
| pEASY ®-Blunt Simple Cloning Vector (10 ng/μl) | 20 μl | 3×20 μl |
| Control Template (5 ng/μl) | 5 μl | 5 μl |
| Control Primers (10 μM) | 5 μl | 5 μl |
| M13 Forward Primer (10 μM) | 50 μl | 150 μl |
| M13 Reverse Primer (10 μM) | 50 μl | 150 μl |
| SR Primer (10 μM) | 50 μl | 150 μl |
| Trans1-T1 Phage Resistant Chemically Competent Cells | 10×100 μl | 30×100 μl |
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