PSIP1 (NM_033222) Human Untagged Clone
Cat# SC119664
Size : 10ug
Contact local distributor :
PSIP1 (NM_033222) Human Untagged Clone
| Product Data | |
| Target Symbol | PSIP1 |
|---|---|
| Synonyms | DFS70; LEDGF; p52; p75; PAIP; PSIP2 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data | Fully Sequenced ORF >OriGene ORF within SC119664 sequence for NM_033222 edited (data generated by NextGen Sequencing) ATGACTCGCGATTTCAAACCTGGAGACCTCATCTTCGCCAAGATGAAAGGTTATCCCCAT TGGCCAGCTCGAGTAGACGAAGTTCCTGATGGAGCTGTAAAGCCACCCACAAACAAACTA CCCATTTTCTTTTTTGGAACTCATGAGACTGCTTTTTTAGGACCAAAGGATATATTTCCT TACTCAGAAAATAAGGAAAAGTATGGCAAACCAAATAAAAGAAAAGGTTTTAATGAAGGT TTATGGGAGATAGATAACAATCCAAAAGTGAAATTTTCAAGTCAACAGGCAGCAACTAAA CAATCAAATGCATCATCTGATGTTGAAGTTGAAGAAAAGGAAACTAGTGTTTCAAAGGAA GATACCGACCATGAAGAAAAAGCCAGCAATGAGGATGTGACTAAAGCAGTTGACATAACT ACTCCAAAAGCTGCCAGAAGGGGGAGAAAGAGAAAGGCAGAAAAACAAGTAGAAACTGAG GAGGCAGGAGTAGTGACAACAGCAACAGCATCTGTTAATCTAAAAGTGAGTCCTAAAAGA GGACGACCTGCAGCTACAGAAGTCAAGATTCCAAAACCAAGAGGCAGACCCAAAATGGTA AAACAGCCCTGTCCTTCAGAGAGTGACATCATTACTGAAGAGGACAAAAGTAAGAAAAAG GGGCAAGAGGAAAAACAACCTAAAAAGCAGCCTAAGAAGGATGAAGAGGGCCAGAAGGAA GAAGATAAGCCAAGAAAAGAGCCGGATAAAAAAGAGGGGAAGAAAGAAGTTGAATCAAAA AGGAAAAATTTAGCTAAAACAGGGGTTACTTCAACCTCCGATTCTGAAGAAGAAGGAGAT GATCAAGAAGGTGAAAAGAAGAGAAAAGGTGGGAGGAACTTTCAGACTGCTCACAGAAGG AATATGCTGAAAGGCCAACATGAGAAAGAAGCAGCAGATCGAAAACGCAAGCAAGAGGAA CAAATGGAAACTGAGCAGCAGAATAAAGATGAAGGAAAGAAGCCAGAAGTTAAGAAAGTG GAGAAGAAGCGAGAAACATCAATGGATTCTCGACTTCAAAGGATACATGCTGAGATTAAA AATTCACTCAAAATTGATAATCTTGATGTGAACAGATGCATTGAGGCCTTGGATGAACTT GCTTCACTTCAGGTCACAATGCAACAAGCTCAGAAACACACAGAGATGATTACTACACTG AAAAAAATACGGCGATTCAAAGTTAGTCAGGTAATCATGGAAAAGTCTACAATGTTGTAT AACAAGTTTAAGAACATGTTCTTGGTTGGTGAAGGAGATTCCGTGATCACCCAAGTGCTG AATAAATCTCTTGCTGAACAAAGACAGCATGAGGAAGCGAATAAAACCAAAGATCAAGGG AAGAAAGGGCCAAACAAAAAGCTAGAGAAGGAACAAACAGGGTCAAAGACTCTAAATGGA GGATCTGATGCTCAAGATGGTAATCAGCCACAACATAACGGGGAGAGCAATGAAGACAGC AAAGACAACCATGAAGCCAGCACGAAGAAAAAGCCATCCAGTGAAGAGAGAGAGACTGAA ATATCTCTGAAGGATTCTACACTAGATAACTAG Clone variation with respect to NM_033222.3 5' Read Nucleotide Sequence >OriGene 5' read for NM_033222 unedited GGTGTTCAGCATTTGTNAATACTACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCAT CCCCGCGCCGCCGCATCCTCCTCGCCGCCTCCCGGNGCTTCGGNACCCCCGGTCTCGCCC CCGAACATGACTCGCGATTTCAAACCTGGAGACCTCATCTTCGCCAAGAGAAGGTTATCC CCATTGGCCAGCTCGAGTAGACGAAGTTCCTGATGGAGCTGTAAAGCCACCCACAAACAA ACTACCCATTTTCTTTTTTGGAACTCATGAGACTGCTTTTTTAGGACCAAAGGATATATT TCCTTACTCAGAAAATAAGGAAAAGTATGGCAAACCAAATAAAAGAAAAGGTTTTAATGA AGGTTTATGGGAGATAGATAACAATCCAAAAGTGAAATTTTCAAGTCAACAGGCAGCAAC TAAACAATCAAATGCATCATCTGATGTTGAAGTTGAAGAAAAGGAAACTAGTGTTTCAAA GGAAGATACCGACCATGAAGAAAAAGCCAGCAATGAGGATGTGACTAAAGCAGTTGACAT AACTACTCCAAAAGCTGCCAGAAGGGGGAGAAAGAGAAAGGCAGAAAAACAAGTAGAAAC TGAGGAGGCAGGAGTAGTGACAACAGCAACAGCATCTGTTAATCTAAAAGTGAGTCCTAA AAGAGGACGACCTGCAGCTACAGAAGTCAAGATTCCAAAACCAAGAGGCAGACCCAAAAT GGGTAAAACAGCCCCTGTCCCTTCAGAAAGTGACATCATTACTTGAAGAGGACAAAAGTA AGTAAAAGGGGGCCAGAGGAACAACCACCTAAAAAGCCGCCCTAGAAAGATGAAAAGGGC CCAGAAAGGAAGAAGAAAACCCAGAAAAAGAGCCGGATAAAAAAGGAGGGAAGCAAGAAG TTGGAATCAAAAAGGAAAAATTA 3' Read Nucleotide Sequence >OriGene 3' read for NM_033222 unedited CGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTGATAGAAAAATCTTTAATGAA AACAAGTTTACACGTTGAACTTATGGCTTAACTAGAATAAATCTAAGTAAGTTAAAACTG TATAAGGACCAAACAGATAATGACTGGTATATATGCAGGTTCTCTCAAAACGGTGATTCC TAGTTCCATTATAAGACCTCTTTAAAAGCAAAAATGCAATTCTGTAGATGAAAACAGTTA TCTCAGAGGGTCAACCACACAATGTCATACAGAGACGCTGGTCTTAAGCCATTTTTTTCC ATATTCATATAATTTCAAAACATGAGAAGTATCCTACTTGCTGAGAAGTGCCAAATGACC CTAATATTCAAAATATCTTAGAAATGCTATCCTTTAGTTAACATACCCTTAAATTTTGTA ACATTGATTTTACCCTGGATTTAGTCCATACACGTCAATGTACAGACTTATCAAAGTACA ATGCTGGAACAACTAGTGTTTGTATTTTTGGAATCTAGAAAATAAAAACAATATAGCCAT GATTTCAAATAGGTGAGTTTCCTTATATAACATTTATTCATATTTATTGTATAAGCATCA TGATTTTTTCTTCCAATTAAGTTTTAGTGACTTCCTAAAGACATTTTTAACAACATTCCT CAAAAATAAACTTACTTAAGTTTTCAGTTCTAGTTACTAGTGCCCTGCTATAAAAGACCT TAAATGATTTAACCCTAAGCCAGATCATTTCTAATATACCCAGTCAGGTGGCTGAAAAGA ATCTTAAGACTTACTGATGCCCACCCCAAAACCTAATACCTTTAAAAATAAGGTCTATAG TTGTAAAATCTAACTCAACGCAGGTGCTAATACGTGGCTTCCCAAAAACCCTTCCTACCC ACTTGTTCCAAAAACTGGTAATGGACCCATTGTAATGGGAAATAA |
| Restriction Sites | NotI-NotI Plasmid Map |
| ACCN | NM_033222 |
| Insert Size | 3100 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@clinisciences.com The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_033222.2, NP_150091.2 |
| RefSeq Size | 3377 bp |
| RefSeq ORF | 1593 bp |
| Locus ID | 11168 |
| UniProt ID | O75475 |
| Cytogenetics | 9p22.3 |
| Domains | PWWP |
| Protein Families | Stem cell - Pluripotency, Transcription Factors |
| Summary | Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR, and 3' coding region, compared to variant 1. This results in a protein (isoform 2) with a longer, distinct C-terminus, compared to isoform 1. Variants 2 and 3 encode the same protein (isoform 2). |