Western blot analysis of LDHA expression in MCF7 (A), Jurkat (B), NIH3T3 (C), mouse skeletal muscle (D) whole cell lysates. (Predicted band size: 26; 30; 36; 39 kD; Observed band size: 37 kD)

Immunofluorescent analysis of LDHA staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.