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> Rat IgA (alpha chain) antibody (Biotin)

Rat IgA (alpha chain) antibody (Biotin)

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Cat# orb347835-1mg

Size : 1mg

Brand : Biorbyt

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Phone : +1 850 650 7790

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    Rat IgA (alpha chain) antibody (Biotin)

    Catalog Number: orb347835

    Catalog Numberorb347835
    CategoryAntibodies
    DescriptionRat IgA (alpha chain) antibody (Biotin)
    Species/HostGoat
    ClonalityPolyclonal
    Tested applicationsELISA, IHC, WB
    ReactivityRat
    IsotypeIgG
    ImmunogenRat IgA alpha heavy chain
    Concentration1.0 mg/mL
    Dilution rangeELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
    Form/AppearanceLyophilized
    PurityThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgA and Rat Serum. No reaction was observed against other Rat heavy or light chain proteins.
    ConjugationBiotin
    StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Buffer/Preservatives0.01% (w/v) Sodium Azide
    Alternative namesGoat anti-Rat IgA (alpha chain) biotin Conjugated
    NoteFor research use only
    Application notesAnti-Rat IgA Biotin Conjugated Antibody has been tested by western blot and assayed against 1.0 ug of Rat IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:30,000 of the reconstitution concentration is suggested for this product.
    Expiration Date12 months from date of receipt.
    Rat IgA (alpha chain) antibody (Biotin)

    Western Blot of Biotin Conjugated Goat Anti-Rat IgA (Alpha chain) Secondary Antibody. Lane 1: Molecular Weight Marker. Lane 2: Mouse IgA Kappa. Load: 100 ng per lane. Primary antibody: Rat IgA (Alpha chain) antibody biotin conjugated at 1:1000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40000 for 30 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Rat IgA. Other band(s): None.