Reagents and instruments for immunology, cell biology and molecular biology.
 
> RAT IgG2a isotype control

RAT IgG2a isotype control

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Cat# ASR2532

Size : 500ug

Brand : Abcepta

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Product Information
Description RAT IgG2a isotype control
Conjugate Unconjugated
Clonality Monoclonal
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
FC
Application Note FlowCytometry 1:1000-1:5000
Physical State Liquid (sterile filtered)
Host Isotype IgG2a
Buffer 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Species of Origin Rat
Stabilizer None
Preservative 0.01% (w/v) Sodium Azide
Additional Information
Shipping Condition Wet Ice
Purity RAT IgG2a isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G.  In an Ouchterlony double diffusion assay the material is non-reactive with antisera to rat IgG1a, IgG1b, IgG3 , IgM , and IgA.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat serum.  Light and heavy chain composition has been confirmed.
Storage Condition Store vial at 4° C prior to opening.   This product is stable 4° C as an undiluted liquid.  Dilute only prior to immediate use.  For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.
Precautions NoteThis product is for research use only and is not intended for therapeutic or diagnostic applications.