This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-1β antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-1β bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-1β in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
- Catalogue No.:
- EM0109
- Alias:
- Interleukin-1 beta ELISA Kit, IL-1 beta ELISA Kit, Catabolin ELISA Kit, IL1B ELISA Kit, IL1F2 ELISA Kit
- Species:
- Mouse
- Range:
- 12.5-800pg/ml (1pg=1100mIU)
- Sensitivity:
- 7.5pg/ml
- Product Name
- Mouse IL-1β(Interleukin 1 Beta) ELISA Kit
- Alias
- Interleukin-1 beta ELISA Kit, IL-1 beta ELISA Kit, Catabolin ELISA Kit, IL1B ELISA Kit, IL1F2 ELISA Kit
- Catalogue No.
- EM0109
- Size
- 48T/96T
- Species
- Mouse
- UniProt ID
- P10749
- Sample Type
- Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
- Detection Method
- Sandwich ELISA, Double Antibody
- Detection Wavelength
- OD450
- Reaction Duration
- 4 hours
- Range
- 12.5-800pg/ml (1pg=1100mIU)
- Sensitivity
- 7.5pg/ml
- Storage
- 2-8°C(Sealed), Don't cryopreserve.
- Specificity
- Specifically binds with IL-1β , no obvious cross reaction with other analogues.
- ELISA Kit Components
Kit Components Item Size(48T) Size(96T) Storage Condition for Opened Kit E001 ELISA Microplate(Dismountable) 8×6 8×12 Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E002 Lyophilized Standard 1vial 2vial Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 6 month at -20°C E003 Biotin-labeled Antibody(Concentrated, 100X) 60ul 120ul 2-8°C (Avoid Direct Light) E034 HRP-Streptavidin Conjugate(SABC, 100X) 60ul 120ul E024 TMB Substrate 5ml 10ml E039 Sample Dilution Buffer 10ml 20ml 2-8°C E040 Antibody Dilution Buffer 5ml 10ml E049 SABC Dilution Buffer 5ml 10ml E026 Stop Solution 5ml 10ml E038 Wash Buffer(Concentrated, 25X) 15ml 30ml E006 Plate Sealer 3 pieces 5 pieces E007 Product Description 1 copy 1 copy - Required Instruments and Reagents
-
- Microplate reader (wavelength: 450nm)
- 37°C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
- Precision single (0.5-10μL, 5-50μL, 20-200μL, 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
- Assay Procedure Summary
-
- Step 1: Add 100ul standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37°C.
- Washing: Wash the plate twice without immersing.
- Step 2: Add 100ul biotin-antibody working solution, seal the plate and statically incubate for 60 minutes at 37°C.
- Washing: Wash the plate three times and immerse for 1min each time.
- Step 3: Add 100ul HRP-Streptavidin Conjugate (SABC) working solution, seal the plate and statically incubate for 30 minutes at 37°C.
- Washing: Wash the plate five times and immerse for 1min each time.
- Step 4: Add 90ul TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)
- Step 5: Add 50ul stop solution. Read at 450nm immediately and calculate.
- Standard Curve
-
This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)
Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.
STD.(pg/ml) OD-1 OD-2 Average 0 0.104 0.11 0.106 12.5 0.142 0.149 0.145 25 0.195 0.205 0.199 50 0.288 0.303 0.294 100 0.444 0.466 0.453 200 0.764 0.803 0.779 400 1.372 1.442 1.4 800 2.05 2.154 2.091 
- Recovery
-
Add a certain amount of IL-1β into the sample. Calculate the recovery by comparing the measured value with the expected amount of IL-1β in the sample.
Sample Type Recovery Range(%) Average(%) serum(n=10) 96-104 99 EDTA plasma(n=10) 94-103 98 Heparin plasma(n=10) 89-96 92 - Linearity
-
Dilute the sample with a certain amount of IL-1β at 1:2, 1:4 and 1:8 to get the recovery range.
Sample Type 1:2 1:4 1:8 serum(n=10) 85-104% 90-105% 85-103% EDTA plasma(n=10) 84-92% 92-101% 86-101% Heparin plasma(n=10) 81-94% 80-91% 81-99% - Precision(%)
-
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Item Intra-assay Precision Inter-assay Precision Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (pg/ml) 25.9 104.2 399 26.2 103.1 408 Standard deviation 1.21 4.89 20.87 1.45 4.84 22.15 CV(%) 4.69 4.69 5.23 5.54 4.69 5.43 - Stability
-
Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
ELISA kit(n=5) 37°C for 1 month 2-8°C for 6 months Average(%) 80 95-100
IF: 17.6
- Journal:
- Journal of Magnesium and Alloys
- Author:
- Department of Neurology, Xuanwu Hospital, Capital Medical University, Beijing, 100053, China
- Sample:
- homogenized brain
- Cited Date:
- 2024-06-28
- Product:
IF: 17.521
- Journal:
- Advanced Science
- Author:
- The People's Hospital of Gaozhou Guangdong Medical University Maoming 525200 China
- Cited Date:
- 2023-04-28
- Product:
IF: 10.723
- Journal:
- Carbohydrate Polymers
- Cited Date:
- 2023-04-21
- Product:
IF: 10.717
- Journal:
- Cell Death & Differentiation
- Cited Date:
- 2021-03-04
- Product:
IF: 10.6
- Journal:
- Journal of Nanobiotechnology
- Author:
- Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, China.
- Sample:
- cell culture supernatants
- Cited Date:
- 2025-01-17
- Product:
IF: 10.6
- Journal:
- Journal of Nanobiotechnology
- Author:
- School of First Clinical Medical, Ningxia Medical University, Yinchuan, 750004, China.
- Sample:
- supernatant
- Cited Date:
- 2025-01-24
- Product:
IF: 9.1
- Journal:
- Pharmacological Research
- Author:
- State Key Laboratory of Organ Failure Research, National Clinical Research Center of Kidney Disease, Guangdong Provincial Clinical Research Center for Kidney Disease, Guangdong Provincial Key Laboratory of Nephrology, Division of Nephrology, Nanfang Hospi
- Cited Date:
- 2024-09-06
- Product:
IF: 8.786
- Journal:
- Frontiers in Immunology
- Author:
- School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China
- Cited Date:
- 2023-06-23
- Product:
IF: 8.2
- Journal:
- International Journal of Biological Macromolecules
- Author:
- College of Food Science and Engineering, Shanxi Agricultural University, Taigu, Shanxi 030801, China; Shanxi Key Laboratory of Edible Fungi for Loess Plateau, Taigu, Shanxi 030801, China.
- Cited Date:
- 2024-04-07
- Product:
IF: 8.1
- Journal:
- Cell Death & Disease
- Author:
- Department of Cardiology, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi, China.
- Cited Date:
- 2024-09-27
- Product:
IF: 7.109
- Journal:
- Cell Death Discovery
- Cited Date:
- 2023-01-29
- Product:
IF: 6.7
- Journal:
- Phytomedicine
- Author:
- School of Pharmacy, Shandong University of Traditional Chinese Medicine, 4655 Daxue Road, Jinan 250355, PR China
- Cited Date:
- 2025-02-28
- Product:
IF: 6.205
- Journal:
- JCI Insight
- Cited Date:
- 2020-11-13
- Product:
IF: 6.064
- Journal:
- Frontiers in Bioengineering and Biotechnology
- Cited Date:
- 2022-09-23
- Product:
IF: 5.895
- Journal:
- Journal of Agricultural and Food Chemistry
- Cited Date:
- 2022-12-15
- Product:
IF: 5.834
- Journal:
- The FASEB Journal
- Cited Date:
- 2023-04-14
- Product:
IF: 5.8
- Journal:
- Biochemical Pharmacology
- Author:
- Shengli Clinical Medical College of Fujian Medical University, Fuzhou, Fujian, China; Fourth Department of Critical Care Medicine, Fujian Provincial Hospital, Fujian Provincial Key Laboratory of Emergency Medicine, Fuzhou, Fujian, China.
- Cited Date:
- 2024-04-07
- Product:
IF: 5.502
- Journal:
- Laboratory Investigation
- Cited Date:
- 2023-01-29
- Product:
IF: 5.33
- Journal:
- Experimental Neurology
- Cited Date:
- 2022-03-25
- Product:
IF: 5.318
- Journal:
- Food Bioscience
- Cited Date:
- 2023-01-28
- Product:
IF: 5
- Journal:
- European Journal of Pharmacology
- Author:
- Department of Pharmacology, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410078, China
- Cited Date:
- 2023-08-11
- Product:
IF: 4.85
- Journal:
- Cellular Signalling
- Author:
- Department of Cardiology, Guangzhou Institute of Cardiovascular Disease, Guangdong Key Laboratory of Vascular Diseases, State Key Laboratory of Respiratory Disease, The Second Affiliated Hospital, Guangzhou Medical University, Guangzhou 510260, China
- Cited Date:
- 2023-05-12
- Product:
IF: 4.847
- Journal:
- Scientific Reports
- Cited Date:
- 2021-01-26
- Product:
IF: 4.8
- Journal:
- International Immunopharmacology
- Author:
- Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Guangzhou, Guangdong, China.
- Cited Date:
- 2024-09-06
- Product:
IF: 4.8
- Journal:
- Nutrients
- Author:
- Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Campus Zacatenco, Unidad Profesional Adolfo López Mateos, Zacatenco, Av. Wilfrido Massieu 399, Colonia Nueva Industrial Vallejo, Alcaldía Gustavo A. Madero, Ciudad de México 07738, M
- Sample:
- supernatant
- Cited Date:
- 2025-02-28
- Product:
IF: 4.8
- Journal:
- Food Bioscience
- Author:
- Department of Neonatology, Affiliated Women's Hospital of Jiangnan University, Wuxi 214002, China
- Cited Date:
- 2024-12-27
- Product:
IF: 4.565
- Journal:
- The Journal of Gene Medicine
- Cited Date:
- 2022-02-10
- Product:
IF: 4.432
- Journal:
- European Journal of Pharmacology
- Cited Date:
- 2022-01-13
- Product:
IF: 4.4
- Journal:
- Molecular and Cellular Endocrinology
- Cited Date:
- 2017-02-16
- Product:
IF: 4.4
- Journal:
- Neurochemical Research
- Author:
- Department of Anesthesiology, Affiliated Hospital of North Sichuan Medical College, Nanchong, China
- Cited Date:
- 2023-09-01
- Product:
IF: 4.1
- Journal:
- Neuroendocrinology
- Author:
- Department of Endocrinology, Renmin Hospital of Wuhan University, Wuchang District, Wuhan, Hubei, China
- Cited Date:
- 2024-01-05
- Product:
IF: 3.9
- Journal:
- Frontiers in Endocrinology
- Author:
- Department of Endocrinology, Renmin Hospital of Wuhan University, Wuhan, China.
- Sample:
- blood samples
- Cited Date:
- 2025-02-07
- Product:
IF: 3.9
- Journal:
- Functional & Integrative Genomics
- Author:
- Department of Nephrology, Chongqing Traditional Chinese Medicine Hospital, No.6 Panxi Road, Jiangbei District, Chongqing, 400021, China.
- Cited Date:
- 2024-08-02
- Product:
IF: 3.9
- Journal:
- RSC Advances
- Author:
- Department of Pharmacognosy, Faculty of Pharmacy, Kafrelsheikh University, P.O. Box 33516, Kafrelsheikh, Egypt
- Cited Date:
- 2023-09-08
- Product:
IF: 3.813
- Journal:
- Prostaglandins & Other Lipid Mediators
- Author:
- Yeditepe University, 26 August Settlement, Atasehir, Istanbul 34755, Turkey
- Cited Date:
- 2023-05-05
- Product:
IF: 3.8
- Journal:
- Acta Diabetologica
- Author:
- Department of Endocrinology and Metabolic Diseases, Dalian University Affiliated Xinhua Hospital, Dalian, 116021, Liaoning, China
- Cited Date:
- 2023-10-20
- Product:
IF: 3.76
- Journal:
- Biomolecules and Biomedicine
- Cited Date:
- 2023-02-16
- Product:
IF: 3.712
- Journal:
- Immunopharmacology and Immunotoxicology
- Author:
- Department of Respiratory Medicine, Baoshan Branch of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China
- Cited Date:
- 2023-07-14
- Product:
IF: 3.7
- Journal:
- Animal Models and Experimental Medicine
- Author:
- Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-Origin Food, Chengdu Medical College, Chengdu, PR China.
- Sample:
- serum
- Cited Date:
- 2024-05-17
- Product:
IF: 3.4
- Journal:
- Cardiovascular Toxicology
- Author:
- Department of Medical Analysis, Princess Aisha Bint Al-Hussein College of Nursing and Health Sciences, Al-Hussein Bin Talal University, Ma'an, 71111, Jordan.
- Cited Date:
- 2025-01-24
- Product:
IF: 3.327
- Journal:
- ??Pancreas
- Cited Date:
- 2021-11-12
- Product:
IF: 3.3
- Journal:
- Toxicology and Applied Pharmacology
- Author:
- Department of Pharmacology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
- Cited Date:
- 2024-09-27
- Product:
IF: 3
- Journal:
- FEBS Letters
- Author:
- Department of Biological Sciences, Bose Institute, Unified Academic Campus, Kolkata, India.
- Sample:
- supernatants
- Cited Date:
- 2024-08-23
- Product:
IF: 2.7
- Journal:
- Tissue and Cell
- Author:
- Department of Medical Analysis, Princess Aisha Bint Al-Hussein College of Nursing and Health Sciences, Al-Hussein Bin Talal University, Ma'an 71111, Jordan.
- Cited Date:
- 2025-03-07
- Product:
IF: 2.5
- Journal:
- Biochemical and Biophysical Research Communications
- Author:
- Institute of Materia Medica, School of Pharmacy, North Sichuan Medical College, Nanchong, 637000, China
- Sample:
- supernatant
- Cited Date:
- 2024-12-06
- Product:
IF: 1.903
- Journal:
- Chemical and Pharmaceutical Bulletin
- Cited Date:
- 2023-04-07
- Product:
IF: 1.7
- Journal:
- Biologicals
- Author:
- Department of Biotechnology, Natural and Applied Sciences Institute, Ege University, 35100, ?zmir, Turkey
- Cited Date:
- 2023-12-22
- Product:
IF: 1.6
- Journal:
- Preventive Nutrition and Food Science
- Author:
- Department of Food Science and Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia.
- Sample:
- supernatant
- Cited Date:
- 2024-10-18
- Product:
- Journal:
- University Giessen
- Cited Date:
- 2017-08-03
- Product:
- Journal:
- Medical Journal of Malaysia
- Author:
- Department of Food Science and Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia
- Sample:
- homogenised colonic tissues
- Cited Date:
- 2024-09-13
- Product:
- Journal:
- Research Square
- Author:
- Nanchang University
- Cited Date:
- 2024-02-02
- Product:
- Journal:
- SSRN
- Author:
- Department of Anesthesiology, the Affiliated Hospital of North Sichuan Medical College, Nanchong, China
- Cited Date:
- 2023-11-03
- Product:
- Journal:
- Research Square
- Author:
- the Affiliated Hospital of North Sichuan Medical College
- Sample:
- hippocampus
- Cited Date:
- 2024-10-25
- Product:
- Journal:
- Research Square
- Author:
- Houston Methodist Research Institute
- Sample:
- serum
- Cited Date:
- 2024-10-25
- Product:
- Journal:
- Research Square
- Cited Date:
- 2021-09-09
- Product: