Recombinant Mouse G-CSF (CHO)

Biological Activity

G-CSF stimulates the bone marrow to produce granulocytes and stem cells, and specifically stimulates the proliferation and differentiation of the neutrophilic granulocyte lineage. G-CSF has been used to stimulate white blood cell production after chemotherapy. It has also been used to boost the number of hematopoietic stem cells after bone marrow transplantation.

Measured in a cell proliferation assay using NFS-60 cells, the ED50 is < 0.02 ng/ml.

Accession #: P09920

Endotoxin: < 0.2 EU/μg

Apparent Molecular Weight: 22~24 kDa, on SDS-PAGE under reducing conditions.

Lyophilized after extensive dialysis against PBS.

Chemical Information

Solubility (25°C)	Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml.
Storage	Stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C.

References

[1] Yuki Endo, et al. Med Oncol. Effect of concomitant use of G-CSF and myelosuppressive chemotherapy on bone marrow and peripheral granulocytes in a mouse model

[2] Li Wang, et al. Radiat Res. PEG-G-CSF and L-Citrulline Combination Therapy for Mitigating Skin Wound Combined Radiation Injury in a Mouse Model

[3] Yuji Guo, et al. Lab Invest. G-CSF promotes autophagy and reduces neural tissue damage after spinal cord injury in mice

[4] Y Ariyoshi. Gan To Kagaku Ryoho. [Recombinant human granulocyte colony-stimulating factor (G-CSF)]

[5] B Perpoint, et al. Exp Hematol. In vitro chronopharmacology of recombinant mouse IL-3, mouse GM-CSF, and human G-CSF on murine myeloid progenitor cells