Reagents and instruments for immunology, cell biology and molecular biology.

Reduced Glutathione (GSH) Colorimetric Assay Kit

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Cat# E-BC-K030-M-500

Size : 500Assays

Contact local distributor :

Phone : +1 850 650 7790

Brand : Elabscience

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Contact local distributor :

Phone : +1 850 650 7790

Detection Principle	Reduced GSH can react with Dinitrobenzoic acid (DNTB) to form a yellow complex which can be detected by colorimetric assay at 405 nm and calculate the reduced GSH content indirectly.
Synonyms	GSH
Sample Type	Serum,Plasma,Cell culture supernatant,Tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (405-414 nm)
Research Area	Oxidative Stress , Cuproptosis , Ferroptosis
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	2 μmol/L
Detection Range	2-100 μmol/L
Precision	inter-assay CV: 3.2% \| intra-assay CV: 1.9%
Assay Time	25 min

Cat.No.	Product Name	Sizes
E-BC-K096-M	Glutathione Peroxidase (GSH-Px) Activity Assay Kit	96T , 48T
E-BC-K097-M	Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit	96T
E-BC-K099-S	Glutathione Reductase (GR) Activity Assay Kit	100Assays , 50Assays
E-BC-K278-S	Glutathione-S-Transferase (GST) Activity Assay Kit	100Assays , 50Assays

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human serum	1
Human plasma	1
10% Mouse brain tissue homogenization	1
10% Mouse liver tissue homogenization	1
Hela cell homogenization (0.999 mgprot/mL)	1
Rat serum	1
Rat plasma	1
Mouse serum	1
10% Carrot tissue homogenization	1
293T supernatant	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.