RL95-2
Brand : CLS Cell Lines Service
RL95-2 Cells
General information
Description | The cells possess alpha keratin, well defined junctional complexes, tonofilaments and surface microvilli. |
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Organism | Human |
Tissue | Uterus, endometrium |
Disease | Endometrial adenosquamous carcinoma |
Synonyms | RL95_2, RL-95-2, RL-952, RL952, RL95 |
Characteristics
Age | 65 years |
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Gender | Female |
Ethnicity | European |
Morphology | Epithelial |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | RL95-2 (Cytion catalog number 305062) |
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Biosafety level | 1 |
Expression / Mutation
Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS, 0.005 mg/ml insulin |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | 1:2 to 1:4 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 10,11 D13S317: 8,12 D16S539: 11,13 D5S818: 10,11 D7S820: 10 TH01: 9,9.3 TPOX: 8 vWA: 16,20 D3S1358: 14,16 D21S11: 28,29 D18S51: 10,14 Penta E: 5,11 Penta D: 9 D8S1179: 10,14 FGA: 20,22 D6S1043: 19 D2S1338: 22,23 D12S391: 15,21 D19S433: 15 |