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> SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag

SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag

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Cat# GTX135357-pro-500ug

Size : 500ug

Brand : GeneTex

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Phone : +1 850 650 7790

SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag. GTX135357-pro
SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag. GTX135357-pro
SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag. GTX135357-pro
SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag. GTX135357-pro
SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag. GTX135357-pro

Application

 ELISA, Sandwich ELISA

Species

 SARS Coronavirus 2
Package
500 μg,
100 μg
Datasheet File
View product citations for antibody GTX135357-pro on CiteAb

APPLICATION

Application Note

Recommended antibody pairs for sandwich ELISA:
Capture: GTX635689, Detection: GTX635686-01 or Capture: GTX135357 / GTX135361, Detection: GTX632269.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

N-terminal His tagged full length protein of YP_009724397.2

Expression System

E. coli

Purity

>90%

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid

DATA IMAGES

SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag. GTX135357-pro

GTX135357-pro ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135357 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag. GTX135357-pro

GTX135357-pro ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag. GTX135357-pro

GTX135357-pro ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135361 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag. GTX135357-pro

GTX135357-pro ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135357-pro) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (HRP) (GTX635686-01) (1 μg/mL)

SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag. GTX135357-pro

GTX135357-pro Image

3μg of GTX135357-pro His tag-tagged SARS-CoV-2 (COVID-19) nucleocapsid protein analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

Application Reference

Lebedin M et al. iScience 2024; 27 (3) : 109330 Soluble ACE2 correlates with severe COVID-19 and can impair antibody responses.
Pin-Hsuan Chen et al. Sens Actuators B Chem 2022; 357 : 131415 Saliva-based COVID-19 detection: A rapid antigen test of SARS-CoV-2 nucleocapsid protein using an electrical-double-layer gated field-effect transistor-based biosensing system.

REVIEW

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Application Reference
Lebedin M et al. iScience 2024; 27 (3):109330 Soluble ACE2 correlates with severe COVID-19 and can impair antibody responses.
Pin-Hsuan Chen et al. Sens Actuators B Chem 2022; 357:131415 Saliva-based COVID-19 detection: A rapid antigen test of SARS-CoV-2 nucleocapsid protein using an electrical-double-layer gated field-effect transistor-based biosensing system.
SDS
PBS.pdf

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