SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL249]
Cat# GTX635678-25ul
Size : 25ul
Brand : GeneTex
Contact local distributor :
Phone : +1 850 650 7790
![Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL249] used in ELISA (ELISA). GTX635678](http://www.genetex.com/templates/default/js/lightbox/images/loading.gif "Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL249] used in ELISA (ELISA). GTX635678")
Host | Rabbit |
|---|---|
Clonality | Monoclonal |
Clone Name | HL249 |
Isotype | IgG |
Application | WB, ICC/IF, ELISA, Sandwich ELISA, IHC-P (cell pellet) |
Reactivity | SARS Coronavirus, SARS Coronavirus 2 |
| Package | 100 μl, 25 μl |
Datasheet File APPLICATION
Application Note
| Application | Recommended Dilution |
|---|---|
| WB | 1:5000-1:20000 |
| ICC/IF | 1:100-1:1000 |
| ELISA | Assay dependent |
| Sandwich ELISA | Assay dependent |
| IHC-P (cell pellet) | Assay dependent |
| Note : Sandwich ELISACapture : GTX635685 / GTX635679 / GTX635680 / GTX635689, Detection : GTX635678. Please notice that GTX635678 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685 / GTX635679 / GTX635680 / GTX635689. Please contact us for custom HRP-conjugated antibody. | |
Product Note
PROPERTIES
Form
Buffer
Preservative
Storage
Concentration
Antigen Species
Immunogen
Purification
Conjugation
RRID
Note
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DATA IMAGES
| | GTX635678 ELISA ImageSandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL. |
| | GTX635678 ELISA ImageIndirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) and (GTX135592-pro) at concentrations ranging from 0.015 μg/mL to 1 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody. |
| | GTX635678 ELISA Image Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL. |
| | GTX635678 ICC/IF ImageSARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920). |
| | GTX635678 ELISA Image Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL. |
| | GTX635678 ELISA Image Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL. |
| | GTX635678 WB ImageNon-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
| | GTX635678 ELISA ImageIndirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.EC50 : 0.016 nM |
| | GTX635678 WB ImageNon-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
| | GTX635678 IHC-P (cell pellet) ImageSARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
| | GTX635678 ICC/IF ImageThe data was published in the 2022 in Viruses. PMID: 35215943 |
Application Reference
REVIEW
| SDS | |
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| PBS.pdf | |