SARS-CoV-2 (COVID-19) Spike antibody
Cat# GTX135360-25ul
Size : 25ul
Brand : GeneTex
Contact local distributor :
Phone : +1 850 650 7790
Host | Rabbit |
|---|---|
Clonality | Polyclonal |
Isotype | IgG |
Application | WB, ICC/IF, ELISA, Sandwich ELISA |
Reactivity | SARS Coronavirus 2 |
| Package | 100 μl, 25 μl |
Datasheet File APPLICATION
Application Note
| Application | Recommended Dilution |
|---|---|
| WB | 1:1000-1:10000 |
| ICC/IF | 1:100-1:1000 |
| ELISA | Assay dependent |
| Sandwich ELISA | Assay dependent |
| Note : Sandwich ELISACapture : GTX632604, Detection: GTX135360 | |
Positive Control
Product Note
PROPERTIES
Form
Buffer
Preservative
Storage
Concentration
Antigen Species
Immunogen
Purification
Conjugation
RRID
Note
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DATA IMAGES
| | GTX135360 WB ImageNon-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) spike antibody (GTX135360) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
| | GTX135360 ICC/IF ImageSARS-CoV-2 (COVID-19) Spike antibody detects SARS-CoV-2 (COVID-19) Spike protein by immunofluorescent analysis.Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SARS-CoV-2 (COVID-19) Spike stained by SARS-CoV-2 (COVID-19) Spike antibody (GTX135360) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920). |
| | GTX135360 ELISA ImageIndirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His and Avi tag (active) protein (GTX01548-pro) (4000-62.5 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike antibody (GTX135360) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody. |
| | GTX135360 WB ImageNon-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike antibody (GTX135360) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
| | GTX135360 WB ImageNon-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike antibody (GTX135360) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. |
| | GTX135360 ELISA ImageSandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX135360 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody. |
| | GTX135360 ELISA ImageIndirect ELISA analysis was performed by coating plate with 50 μL of recombinant full-length SARS-CoV-2 (COVID-19) spike protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) spike antibody (GTX135360) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody. |
Application Reference
REVIEW
| SDS | |
|---|---|
| PBS.pdf | |
| Glycerol.pdf | |
| Proclin.pdf | |