SK-HEP-1 growing culture
Brand : CLS Cell Lines Service
SK-HEP-1 Cells
General information
Description | The SK-HEP-1 cell line is a cancer cell line derived from a liver adenocarcinoma in a 52-year old Caucasian man. It has been shown to form tumors in immunocompromised mice, produce fibronectin, alpha-1 protease inhibitor, and Interleukin-1. However, there is an alternative hypothesis that the cells are of endothelial origin and not hepatocytes. |
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Organism | Human |
Tissue | Liver |
Disease | Adenocarcinoma |
Metastatic site | Ascites, endothelial cells |
Synonyms | SK-Hep-1, SK HEP-1, SK HEP 01, SK-Hep1, Sk-Hep1, SK Hep1, SKHEP-1, SKHEP1, SKHep1, SK_HEP1 |
Characteristics
Age | 52 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SK-HEP-1 (Cytion catalog number 300334) |
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Biosafety level | 1 |
Expression / Mutation
Isoenzymes | Me-2, 1-2, PGM3, 1, PGM1, 2, ES-D, 1, AK-1, 1, GLO-1, 1, G6PD, B |
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Tumorigenic | Yes, in nude mice, forms large cell carcinoma consistent with hepatoma |
Karyotype | (P11) hyperdiploid to hypotriploid (+A3, +C, +E, +F, +G, -A, -D) with abnormalities including dicentrics, acrocentric fragments, secondary constrictions, pulverizations, and large subtelocentric and submetacentric markers |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile | Amelogenin: x,x CSF1PO: 11,12 D13S317: 8,12 D16S539: 12 D5S818: 10,13 D7S820: 8,11 TH01: 7,9 TPOX: 9 vWA: 14,17 D3S1358: 16 D21S11: 29,31 D18S51: 13,15 Penta E: 13,21 Penta D: 13,14 D8S1179: 13,14 FGA: 17 D1S1656: 16,17 D6S1043: 11 D2S1338: 20,23 D12S391: 18 D19S433: 12,15.2 |
HLA alleles | A*: 02:01:01, 24:02:01 B*: 35:02:01, 44:03:01 C*: 04:01:01 DRB1*: 10:01:01, 11:04:01 DQA1*: 01:05:01, 05:05:01 DQB1*: 03:01:01, 05:01:01 DPB1*: 04:01:01 E: 01:01, 01:03 |