Application
| WB, IHC, ICC, E, AM |
|---|---|
| Primary Accession | P60880 |
| Other Accession | NP_570824.1 |
| Host | Mouse |
| Isotype | IgG1 |
| Reactivity | Human, Mouse, Rat, Pig, Cat |
| Clonality | Monoclonal |
| Description | Mouse Anti-Human SNAP-25 Monoclonal IgG1 |
| Target/Specificity | Detects ~29kDa. |
| Other Names | Bdr Antibody, RIC4 Antibody, SNAP25 Antibody, SNAP-25B Antibody, SNP25 Antibody, SUP Antibody, super protein Antibody, synaptosomal associated 25kDa protein Antibody |
| Clone Names | SP-12 |
| Immunogen | Raised against a crude synaptic preparation from the post mortem human brain |
| Purification | Protein G Purified |
| Storage | -20ºC |
| Storage Buffer | PBS pH7.4, 50% glycerol, 0.09% sodium azide |
| Shipping Temperature | Blue Ice or 4ºC |
| Certificate of Analysis | 1 µg/ml of SMC-180 was sufficient for detection of SNAP-25 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
| Cellular Localization | Cytoplasm |
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Provided below are standard protocols that you may find useful for product applications.
Background
Synaptosomal-associated protein 25 (SNAP-25) is a membrane bound protein anchored to the cytosolic face of membrane via palmitoyl side chains in the middle of the molecule (1). It is a component of the SNARE complex, which is proposed to account for the specificity of membrane fusion and to directly execute fusion by forming a tight complex that brings the synaptic vesicle and plasma membranes together (2). SNAP25 exists as two alternatively spliced isoforms, SMAP25A and SNAP25B. These isoforms are differentially expressed in neurons and neuroendocrine cells and they differ by 9 amino acids in the central portion of the protein (3).
References
1. Hodel A. (1998) Int J. Biochem Cell Biol. 30(10): 1069-1073.
2. Sudhof T.C., and Rizo J. (2002) Nat Rev Neurosci. 3(8): 641-653.
3. Puffer E.B., et al. (2001) Biochemistry. 40(31): 9374-9378.