STRO-1 (A Stromal Cell Precursor Surface Antigen)

STRO-1 is a cell surface protein expressed by bone marrow stromal cells and erythroid precursors. The frequency of colony forming units-fibroblasts (CFU-F) was enriched 100-fold in the STRO-1+/Glycophorin A- population from bone marrow cells (1). The subset of marrow cells that expresses the STRO-1 antigen is capable of differentiating into multiple mesenchymal lineages including hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype, adipocytes, osteoblasts, and chondrocytes (2).||Applications: |Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested.||Recommended Dilutions:|Flow Cytometry: 2.5ug/10e6 cells with MG‑63 human osteosarcoma cell line|Immunocytochemistry: 8­-25ug/ml in immersion fixed MG-63 human osteosarcoma cell line|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.