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> TLR4 ELISA Kit (Rat)

TLR4 ELISA Kit (Rat)

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Cat# OKEH00569

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00569
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology TLR4 ELISA Kit (Rat) (OKEH00569) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TLR4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TLR4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TLR4 captured in well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.8% (n = 20)
Mean Inter-assay CV%: < 8.1% (n = 20)
ELISA Kit Component
ComponentAmount
Anti-TLR4 Microplate96 Wells (12 x 8 Well strips)
TLR4 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated TLR4 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.159 ng/mL
SpecificityNatural and recombinant Rat Toll-like receptor 4
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolTlr4
Gene Full Nametoll-like receptor 4
Alias Symbolstoll4;toll-like receptor 4.
NCBI Gene Id29260
Protein NameQ9QX05
Description of TargetToll-like receptor 4
Uniprot IDhttps://www.uniprot.org/uniprot/Q9QX05
Protein Accession #NP_003257.1
Nucleotide Accession #NM_003266.3
Protein Size (# AA)839
Molecular Weight95 kDa
Biological pathways
Name# of Products
Activation of MAPK activity317
Cellular response to mechanical stimulus218
Defense response to Gram-negative bacterium93
Detection of fungus14
Detection of lipopolysaccharide19
I-kappaB phosphorylation65
Innate immune response905
Intestinal epithelial structure maintenance20
MyD88-dependent toll-like receptor signaling pathway331
MyD88-independent toll-like receptor signaling pathway288
Negative regulation of ERK1 and ERK2 cascade59
Negative regulation of interferon-gamma production55
Negative regulation of interleukin-17 production68
Negative regulation of interleukin-23 production16
Negative regulation of interleukin-6 production98
Negative regulation of osteoclast differentiation65
Negative regulation of tumor necrosis factor production93
Positive regulation of chemokine production128
Positive regulation of inflammatory response172
Positive regulation of interferon-alpha production52
Positive regulation of interferon-beta production86
Positive regulation of interferon-gamma production108
Positive regulation of interleukin-1 production16
Positive regulation of interleukin-10 production69
Positive regulation of interleukin-12 biosynthetic process28
Positive regulation of interleukin-12 production171
Positive regulation of interleukin-6 production149
Positive regulation of interleukin-8 biosynthetic process38
Positive regulation of interleukin-8 production127
Positive regulation of NF-kappaB import into nucleus114
Positive regulation of NF-kappaB transcription factor activity346
Positive regulation of nitric-oxide synthase biosynthetic process110
Positive regulation of platelet activation30
Positive regulation of transcription from RNA polymerase II promoter1210
Positive regulation of tumor necrosis factor biosynthetic process63
Positive regulation of tumor necrosis factor production168
T-helper 1 type immune response37
Toll signaling pathway369
Toll-like receptor 1 signaling pathway309
Toll-like receptor 2 signaling pathway309
Toll-like receptor 4 signaling pathway330

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