Reagents and instruments for immunology, cell biology and molecular biology.
 
> TMB (dihydrochloride) [64285-73-0]

TMB (dihydrochloride) [64285-73-0]

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Cat# HY-15930A-5g

Size : 5g

Brand : MedChemExpress

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TMB dihydrochloride is the dihydrochloride form of TMB (HY-15930). TMB dihydrochloride is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB dihydrochloride can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB dihydrochloride can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922.

For research use only. We do not sell to patients.

TMB dihydrochloride Chemical Structure

TMB dihydrochloride Chemical Structure

CAS No. : 64285-73-0

This product is a controlled substance and not for sale in your territory.

Based on 1 publication(s) in Google Scholar

Other Forms of TMB dihydrochloride:

  • TMB In-stock
  • TMB monosulfate In-stock
  • TMB dihydrochloride x.hydrate Get quote
  • TMB dihydrochloride hydrate Get quote
Description

TMB dihydrochloride is the dihydrochloride form of TMB (HY-15930). TMB dihydrochloride is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB dihydrochloride can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB dihydrochloride can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922[1][2].
In Vitro

TMB dihydrochloride can tightly bind to Escherichia coli ATCC 25922 cells instead of being internalized to detect singlet oxygen produced by the outer membrane[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TMB dihydrochloride Related Antibodies
Molecular Weight

313.27

Formula

C16H22Cl2N2
CAS No.

64285-73-0
Appearance

Solid

Color

White to off-white

SMILES

NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C.[2HCl]
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 33 mg/mL (105.34 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : 11.11 mg/mL (35.46 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1921 mL 15.9607 mL 31.9213 mL
5 mM 0.6384 mL 3.1921 mL 6.3843 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.83%

COA (248 KB) HNMR (157 KB) LCMS (309 KB)

Handling Instructions (2659 KB)
References

  • [1]. Bresolí-Obach R, et al. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors (Basel). 2020 Oct 21;20(20):5952.  [Content Brief]

    [2]. Frey A, et al. A stable and highly sensitive 3,3',5,5'-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. J Immunol Methods. 2000 Jan 13;233(1-2):47-56.  [Content Brief]

  • [1]. Bresolí-Obach R, et al. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors (Basel). 2020 Oct 21;20(20):5952.

    [2]. Frey A, et al. A stable and highly sensitive 3,3',5,5'-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. J Immunol Methods. 2000 Jan 13;233(1-2):47-56.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.1921 mL 15.9607 mL 31.9213 mL 79.8034 mL
5 mM 0.6384 mL 3.1921 mL 6.3843 mL 15.9607 mL
10 mM 0.3192 mL 1.5961 mL 3.1921 mL 7.9803 mL
15 mM 0.2128 mL 1.0640 mL 2.1281 mL 5.3202 mL
20 mM 0.1596 mL 0.7980 mL 1.5961 mL 3.9902 mL
25 mM 0.1277 mL 0.6384 mL 1.2769 mL 3.1921 mL
30 mM 0.1064 mL 0.5320 mL 1.0640 mL 2.6601 mL
DMSO 40 mM 0.0798 mL 0.3990 mL 0.7980 mL 1.9951 mL
50 mM 0.0638 mL 0.3192 mL 0.6384 mL 1.5961 mL
60 mM 0.0532 mL 0.2660 mL 0.5320 mL 1.3301 mL
80 mM 0.0399 mL 0.1995 mL 0.3990 mL 0.9975 mL
100 mM 0.0319 mL 0.1596 mL 0.3192 mL 0.7980 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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TMB dihydrochloride Related Classifications

  • Others
  • Others NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  • Fluorescent Dye Reactive Oxygen Species
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

TMB64285-73-0BM blueSure Blue TMBFluorescent DyeReactive Oxygen Speciesdyestuffchromogenic agentsinglet oxygenprobeInhibitorinhibitorinhibit