TNF-alpha (APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor) (PE)

Cat# 168877-AF-PE-100ul

Size : 100ul

Brand : US Biological

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168877-AF-PE TNF-alpha (APC1, Cachectin, Differentiation inducing factor (DIF), Macrophage cytotoxic factor (MCF), Necrosin, TNF alpha, TNF Macrophage Derived, TNF Monocyte Derived, TNF Superfamily Member 2, TNFA, TNFSF2, Tumor necrosis factor ligand superfamily member 2, Tumor Necrosis Factor Precursor) (PE)

Clone Type
Polyclonal
Host
mouse
Source
human
Swiss Prot
P01375 (Human)
Isotype
IgG1,k
Grade
Purified
Applications
IF IHC IP
Crossreactivity
Ca Fe Hu Mo Rb Rt Ze
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.||Applications: |Suitable for use in Immunofluorescence, ELISA, Flow Cytometry (Not Tested), Immunoprecipitation, Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Flow Cytometry (Not Tested): 0.5-1ug/million cells |Immunofluorescence: 1-2ug/ml |Immunoprecipitation: 1-2ug/500ug protein |Immunohistochemistry: Frozen & Formalin-paraffin: 1-2ug/ml for 30 min at RT|(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Optimal dilutions to be determined by the researcher.||Positive Control:|HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil||Storage and Stability:|Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.||Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1,k|Clone No: 4C6-H8|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).|Purity: Purified by Protein A/G affinity chromatography.|Immunogen: A hexadecapeptide corresponding to aa115-130 of human TNF-alpha, conjugated to thyroglobulin. Cellular Localization: Cytoplasmic and extracellular (secreted)|Specificity: Recognizes human TNF-alpha. Species Crossreactivity: mouse, rat, rabbit, feline, canine, and zebrafish. Others-not known.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
A hexadecapeptide corresponding to aa115-130 of human TNF-alpha, conjugated to thyroglobulin. Cellular Localization: Cytoplasmic and extracellular (secreted)
Form
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with R-Phycoerythrin (PE).
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human TNF-alpha. Species Crossreactivity: mouse, rat, rabbit, feline, canine, and zebrafish. Others-not known.
References
1. Bebok Z; Markus B; Nemeth P. Prognostic relevance of transforming growth factor alpha (TGF-alpha) and tumor necrosis factor alpha (TNF-alpha) detected in breast cancer tissues by immunohistochemistry. Breast Cancer Research and Treatment, 1994, 29(3):229-35. 2. Bebok Z; Szekeres G; Horvath G; Duda E; Nemeth P. [Creation of monoclonal antibodies against tumor necrosis factor-alpha (TNF-alpha) and transforming growth factor alpha (TFG-alpha), their definition and possible use]. Orvosi Hetilap, 1993 Jun 13, 134(24):1303-7. Language: Hungarian.