TransStart® Tip Green qPCR SuperMix (+Dye II )
Cat# AQ142-22
Size : 5×1ml
Brand : TransGen Biotech
Contact local distributor :
Phone : +1 850 650 7790
ManualTransStart® Tip Green qPCR SuperMix is a ready-to-use qPCR cocktail. It contains a novel TransStart® TipTaq DNA Polymerase,unique hot start reagents (DNA binding proteins combined with unique chemical), optimized double cation buffer, EvaGreen I, dNTPs, PCR Enhancerm and PCR stabilizer. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primer, passive reference dye (optional) and Nuclease-free Water.
Highlights
• A combination of chemical blocking technique with TransStart® hot start technique to achieve complete blocking. Compared with double blocking TransStart®TopTaq, this method provides higher sensitivity, higher specificity, better amplification.
• Double cation (K+, NH4+) buffer enhances specificity and reduces primer-dimer formation.
• Passive reference dyes are provided for different qPCR instruments.
Storage
at -20°C in dark for two years
Shipping
Dry ice (-70 ℃)
| Component | AQ141-01 | AQ141-02 | AQ141-03 | AQ141-04 |
| 2×TransStart® Tip Green qPCR SuperMix | 1 ml | 5×1 ml | 15×1 ml | 25×1 ml |
| Passive Reference Dye (50×) | 40 μl | 200 μl | 600 μl | 1 ml |
| Nuclease-free Water | 1 ml | 5 ml | 3×5 ml | 5×5 ml |
Wei Y , Hu W , Wang Q , et al. Identification, transcriptional and functional analysis of heat-shock protein 90s in banana (Musa acuminata L.) highlight their novel role in melatonin-mediated plant response to Fusarium wilt[J]. Journal of Pineal Research, 2017, 62(1):e12367.
Wei Q , Jiao C , Guo L , et al. Exploring key cellular processes and candidate genes regulating the primary thickening growth of Moso underground shoots[J]. New Phytologist, 2017, 214(1):81-96.
Qi F , Yang J , Xia Y , et al. A(H1N1) vaccination recruits T lymphocytes to the choroid plexus for the promotion of hippocampal neurogenesis and working memory in pregnant mice[J]. Brain Behavior & Immunity, 2016, 53:72-83.
Liu G , Zhang K , Ai J , et al. Patatin-related phospholipase A, pPLAIIIα, modulates the longitudinal growth of vegetative tissues and seeds in rice[J]. Journal of Experimental Botany, 2015, 66(21):6945-6955.
Li P , Shi M L , Shen W L , et al. Coordinated regulation of IFITM1, 2 and 3 genes by an IFN-responsive enhancer through long-range chromatin interactions[J]. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2017, 1860(8).