Reagents and instruments for immunology, cell biology and molecular biology.
 
> TrueBlot® Protein G Magnetic Beads IP/Co-IP Kit

TrueBlot® Protein G Magnetic Beads IP/Co-IP Kit

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Cat# KBA-PG00-18

Size : 1Kit

Brand : Rockland Immunochemicals

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Specifications for Protein G Magnetic Beads IP/Co-IP Kit

Product Details

TrueBlot® Protein G Magnetic Beads IP/Co-IP Kit - KBA-PG00-18
Magnetic beads, particles, microparticles, paramagnetic nanoparticles, magnets, immunoprecipitation, protein antibody isolation, purification, IgG
Protein G

Application Details

IP
TrueBlot® Protein G magnetic beads IP/Co-IP kit can be used for immunoprecipitation (IP) and Co-IP experiments as well as for antibody purification. For IP, target specific antibody is incubated with cell lysate. Protein G magnetic beads are then incubated with antigen-antibody complex at room temperature, washed and then eluted using elution buffer. The samples are then resolved by SDS-PAGE and analyzed by Western blotting. For antibody purification, Protein G magnetic beads are incubated with the antibody solution and then separated by magnets. After the unbound particulates are washed from the beads, the bound antibodies are eluted from the beads using the elution buffer. The beads are then magnetically separated from the eluted solution, which is removed manually.

Formulation

5.0mg/mL by UV absorbance at 280 nm

Shipping & Handling

Wet Ice
The Protein G Magnetic Beads should be stored in the refrigerator (2-8 °C). The reagent must be allowed to reach room temperature (20-25 °C) before use and may be used until the expiration date. Do not freeze, dry, or centrifuge the beads as they may result in loss of binding activity and aggregation. See kit insert for storage components.
Expiration date is one (1) year from date of receipt.

Background

TrueBlot® Protein G Magnetic Beads IP/Co-IP Kit allows highly effective and efficient immunoprecipitation (IP) and co-immunoprecipitation (co-IP) of an antigen using smaller amounts of antibody (typically 1-10 µg). The kit is designed for immunoprecipitation of proteins, protein complexes, protein-nucleic acid complexes and other antigens. The specific antibody is added to the sample to form an immune complex. The immune complex is then bound to Protein G Magnetic beads. The complex is washed to remove unbound material and the bound immune complex is then eluted from the beads using low-pH elution buffer. Alternatively, the sample buffer is used for dissociation using denaturing conditions or for down-stream process for SDS-PAGE analysis. The kit includes Protein G magnetic beads for high capture efficiencies and optimized buffers for high yield of target antigen. Bead mean diameter is ~0.5 μm, bead concentration is 5 mg/mL. The kit contains magnetic beads, Binding/Wash buffer, Elution buffer, and Neutralization buffer sufficient for 20 samples or tests. (See protocol for details). The beads are removed from the solution using a magnetic separator.

Related Protocols

Adherent Cell Lysis Protocol
Immunoprecipitation (IP) Protocol
IP-WB with TrueBlot® Protocol
Suspension Cultured Cell Lysis Protocol

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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