Tumor Necrosis Factor Receptor 1, p55 (TNFR 1, CD120a, MGC19588, p55, p55 R, TNFR55, Tumor Necrosis Factor alpha Receptor, Tumor Necrosis Factor Binding Protein 1, TBP1, Tumor Necrosis Factor Receptor Superfamily Member 1A, TNFRSF1a)

Cat# T9161-06G-100ug

Size : 100ug

Brand : US Biological

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T9161-06G Tumor Necrosis Factor Receptor 1, p55 (TNFR 1, CD120a, MGC19588, p55, p55 R, TNFR55, Tumor Necrosis Factor alpha Receptor, Tumor Necrosis Factor Binding Protein 1, TBP1, Tumor Necrosis Factor Receptor Superfamily Member 1A, TNFRSF1a)

Clone Type
Polyclonal
Host
rabbit
Source
mouse
Swiss Prot
P25118
Isotype
IgG
Grade
Affinity Purified
Applications
E IHC IP WB
Crossreactivity
Bo Ca Dr Hm Hu Mk Mo Po Rb Rt Sh Xe Ye
Accession #
X59238
Shipping Temp
Blue Ice
Storage Temp
-20°C

Tumor necrosis factor alpha (TNFalpha), also known as cachectin, is a 17.5kD, 157 amino acid member of the TNF superfamily of cytokines that is a potent lymphoid factor with effects on a wide range of target cells. Active TNFalpha is produced in both soluble and membrane-anchored trimers by macrophages, NK cells, and T- and B-lymphocytes. TNF exerts proinflammatory signals via binding and inducing trimerization of TNF-receptor 1 (TNFR 1) expressed on most normal and transformed cells, or to TNF-receptor 2 (TNFR 2), expressed on endothelial and most immune cells. TNF signaling regulates hematopoiesis, differentation, endothelial cell activation, apoptosis, lipid metabolism, tumor progression, and immune suveillance, and dysregulation of TNF or its receptors is implicated in numerous disease states including cancer, osteoporosis, autoimmune disease, diabetes, and atherosclerosis.||Applications:|Suitable for use in ELISA, Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Western Blot: 1:1000 using ECL|Immunoprecipitation: 12.5ug/ml|Immunohistochemistry (paraffin): 10ug/ml|Optimal dilutions to be determined by the researcher.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: ~1mg/ml|Form: Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol. |Purity: Purified by peptide affinity chromatography.|Immunogen: Synthetic peptide corresponding to a portion of mouse Tumor Necrosis Factor Receptor 1, p55 (TNFR 1).|Species Sequence Homology: rat and human; 100%|Specificity: Recognizes mouse TNFR 1 at ~55kD. Species Crossreactivity: rat, human, hamster, bovine, rabbit, monkey, sheep, canine, porcine, Drosophila, Xenopus and yeast. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to a portion of mouse Tumor Necrosis Factor Receptor 1, p55 (TNFR 1).|Species Sequence Homology: rat and human; 100%
Form
Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.
Purity
Purified by peptide affinity chromatography.
Specificity
Recognizes mouse TNFR 1 at ~55kD. Species Crossreactivity: rat, human, hamster, bovine, rabbit, monkey, sheep, canine, porcine, Drosophila, Xenopus and yeast.
References
1. Hartz, A.M.S., et al., FASEB Journal 22: 2723-2733 (2008). General References: 1. GenBank Accession#: X59238. 2. Dembic, Z., et al., Cytokine 4: 231-237 (1990). 3. Chen, G. & Goeddel, D.V., Science 296(5573): 1634-1635 (2002). 4. Tartaglia, L.A., et al., Cell 74: 845-853 (1993). 5. Yuasa, T., et al., J. Biol. Chem. 28: 22,681-22,692 (1998). 6. Guo, D., et al., J. Immunol. 160(6): 2742-2750 (1998). 7. Yamawaki, H., et al., Circulation 108(13): 1619-1625 (2003). 8. Castaneda, M.P., et al., Transplantation 76(1): 50-54 (2003). 9. Rao, N., et al., Am. J. Opthamol. 146: 866 (2008). 10. Berk, B.C., et al., Circulation 108: 1619 (2003).