Western Blot Stripping Buffer (Neutral)
Cat# E-IR-R102-500
Size : 500mL
Brand : Elabscience
Contact local distributor :
Phone : +1 850 650 7790
| Product Introduction | Western Blot Stripping Buffer is used for the reuse of the transferred protein membrane in Western blot. After primary antibody binding to the membrane and secondary antibody binding to the primary antibody, then using ECL subsequent to exposure the images, if the samples on the membrane are also needed to verify some control proteins or other target proteins, this product can be used to elute the combined antibodies before. After the antibody signals are cleared, the membrane can be re-incubated with control antibodies or other antibodies for the second verification. It can save the time of preparing SDS-PAGE gel and transfer, and the samples of experiment are also saved. It only takes about 30 min to reuse the protein membrane which can eliminate the error caused by redoing the experiment and makes the experiment results more comparable. |
| Applications | WB |
| Storage | Store at 2~8°C for 12 months. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| Cautions | 1. When the PVDF membrane is immersed with this product, the membrane will become transparent, which is a normal phenomenon. 2. In order to obtain the best performance, PVDF membrane is recommended for this product. 3. This product is recommended to ECL or similar chemiluminescent reagents. If non-chemiluminescent reagents, such as DAB is used to expose the image, please don’t use this product. 4. Repeated use of the same membrane with western blot experiments may lead to the weakening of protein signal. The membrane can be reused 1~2 times with several antibodies tests. 5. Repeated washing the PVDF membrane may lead to weak signal, please detect the lower expression protein first, and higher proteins such as control after cleaning with Western Blot Stripping Buffer. 6. For your safety and health, please wear experimental clothes and disposable gloves. |
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