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> WST-1 [150849-52-8]

WST-1 [150849-52-8]

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Cat# HY-136976-100mg

Size : 100mg

Brand : MedChemExpress

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WST-1 is a kind of water-soluble tetrazolium salt. WST induces the intracellular mitochondrial dehydrogenase to conduct NADH-dependent enzyme digestion reaction, releasing the water-soluble methyl benzene product. WST-1 can be used for the detection of cell proliferation and cytotoxicity, via the determination of the light absorption value at 450 nm.

For research use only. We do not sell to patients.

WST-1 Chemical Structure

WST-1 Chemical Structure

CAS No. : 150849-52-8

This product is a controlled substance and not for sale in your territory.

Based on 2 publication(s) in Google Scholar

Description

WST-1 is a kind of water-soluble tetrazolium salt. WST induces the intracellular mitochondrial dehydrogenase to conduct NADH-dependent enzyme digestion reaction, releasing the water-soluble methyl benzene product. WST-1 can be used for the detection of cell proliferation and cytotoxicity, via the determination of the light absorption value at 450 nm[1].

In Vitro

Preparation of WST-1 working solution
1.1 Preparation of the stock solution
Dissolve 10 mg of WST-1 in 1 mL of DMSO to obtain 10 mg/mL of WST-1.
1.2 Preparation of WST-1 working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 5-10 μM of WST-1 working solution.
Note: Please adjust the concentration of WST-1 working solution according to the actual situation.

Cell staining
2.1 Cell preparation.
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2.2 Add 1 mL of WST-1 working solution and 1-methoxy PMS (2 mM), and then incubate at room temperature for 2 h.
2.3 Use microplate reader detect 450/690 nm OD.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

WST-1 Related Antibodies
Molecular Weight

651.34

Formula

C19H11IN5NaO8S2
CAS No.

150849-52-8
Appearance

Solid

Color

Light yellow to brown

SMILES

O=[S]([O-])(C1=C(C=CC([S](=O)(O[Na])=O)=C1)C2=NN(C(C=C3)=CC=C3I)[N+](C(C=C4)=CC=C4[N+]([O-])=O)=N2)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 125 mg/mL (191.91 mM; Need ultrasonic)

DMSO : 25 mg/mL (38.38 mM; ultrasonic and warming and heat to 80°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5353 mL 7.6765 mL 15.3530 mL
5 mM 0.3071 mL 1.5353 mL 3.0706 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (3.84 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (3.84 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation

Purity: ≥98.0%

COA (257 KB) HNMR (143 KB)

Handling Instructions (2659 KB)
References

  • [1]. Munetaka ISHIYAMA, et al. A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dye. Chem. Pharm. Bull. 1993, 41(6): 1118-1122.

  • [1]. Munetaka ISHIYAMA, et al. A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dye. Chem. Pharm. Bull. 1993, 41(6): 1118-1122.

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO / H2O 1 mM 1.5353 mL 7.6765 mL 15.3530 mL 38.3824 mL
5 mM 0.3071 mL 1.5353 mL 3.0706 mL 7.6765 mL
10 mM 0.1535 mL 0.7676 mL 1.5353 mL 3.8382 mL
15 mM 0.1024 mL 0.5118 mL 1.0235 mL 2.5588 mL
20 mM 0.0768 mL 0.3838 mL 0.7676 mL 1.9191 mL
25 mM 0.0614 mL 0.3071 mL 0.6141 mL 1.5353 mL
30 mM 0.0512 mL 0.2559 mL 0.5118 mL 1.2794 mL
H2O 40 mM 0.0384 mL 0.1919 mL 0.3838 mL 0.9596 mL
50 mM 0.0307 mL 0.1535 mL 0.3071 mL 0.7676 mL
60 mM 0.0256 mL 0.1279 mL 0.2559 mL 0.6397 mL
80 mM 0.0192 mL 0.0960 mL 0.1919 mL 0.4798 mL
100 mM 0.0154 mL 0.0768 mL 0.1535 mL 0.3838 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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WST-1 Related Classifications

  • Cancer
  • Cancer Immunotherapy Cancer Metabolism and Metastasis
  • Others
  • Fluorescent Dye
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

WST-1150849-52-8WST1WST 1Fluorescent DyeformazancellviabilityNADPHmitochondrialdehydrogenaseInhibitorinhibitorinhibit

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