Yeast Vitality Staining Kit (Calcofluor White and ViaVac Red/Green)
Cat# 31062
Size : 1kit
Brand : Biotium
Yeast Vitality Staining Kit
A fluorogenic assay that allows you to determine whether your yeast cells are metabolically active. Contains two fluorescent components, Calcofluor White and ViaVac™ Red/Green.
Product Description
The Yeast Vitality Staining Kit is a fluorogenic assay that allows you to determine whether your yeast cells are metabolically active. The kit contains two fluorescent components: Calcofluor White and ViaVac™ Red/Green.
The kit has been validated in the following species: Saccharomyces cerevisiae, Candida albicans, Aspergillus niger, and Zygosaccharomyces bailii.
Features
- For live-cell imaging of yeast and fungi
- ViaVac™ Red/Green stains the vacuole red in metabolically active cells
- Calcofluor White labels all cell walls blue
Kit Components
- ViaVac™ Red/Green, 10 mM in DMSO, 100 uL
- Calcofluor White, 5 mM in water, 500 uL
Spectral Properties
- ViaVac™ Red/Green Ex/Em ~485/620 nm (red); 485/530 nm (green)
- Calcofluor White Ex/Em 360/430 nm
How It Works
ViaVac™ Red/Green is a fluorescent viability dye for yeast, identical in structure to the dye FUN® 1. ViaVac passively diffuses into cells, showing green cytoplasmic staining. In many species of yeast and fungi, in metabolically active cells the dye is actively transported into the vacuole, resulting in red fluorescent staining of tubular intravacuolar structures.
Calcofluor White is a fluorescent blue dye that binds to cellulose and chitin, which can be found in the cell walls of fungi, algae, and plants.
After staining yeast with this kit, both living and dead cells will be outlined in blue, while only living cells will show red fluorescent, vacuolar tubules.