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> Zebrafish DA(Dopamine) ELISA Kit

Zebrafish DA(Dopamine) ELISA Kit

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Cat# orb1088183-96

Size : 96Test

Brand : Biorbyt

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Phone : +1 850 650 7790

    Zebrafish DA(Dopamine) ELISA Kit

    Catalog Number: orb1088183

    Catalog Numberorb1088183
    CategoryAssays and Kits
    DescriptionThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dopamine(DA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dopamine(DA). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dopamine(DA) in the samples is then determined by comparing the OD of the samples to the standard curve.
    ReactivityZebrafish
    Range15.63-1000 pg/mL
    Concentration1000 pg/mL
    Sensitivity4.71 pg/mL
    Protein Length2h
    Alternative names2-(3,4-Dihydroxyphenyl)ethylamine, 3,4-Dihydroxyph
    NoteFor research use only
    Application notesStandard: 1000 pg/mL. Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish DA protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish DA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish DA in the samples is then determined by comparing the OD of the samples to the standard curve
    Sample Typesserum, plasma and other biological fluids
    Expiration DatePlease enquire.
    Zebrafish DA(Dopamine) ELISA Kit