Cell Biology
1. Introduction :
Liver fibrosis is a chronic condition characterized by the progressive scarring of liver tissue caused by ongoing liver damage. The excessive accumulation of extracellular matrix (ECM) in the liver can result from various factors, such as viral infections, alcohol consumption, metabolic dysfunction-associated liver disease, cholestatic liver disease, and autoimmune hepatitis.
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2D v 3D Cell Culture
The benefits of 3D cell culture over conventional 2D culture are widely accepted in the scientific community.
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In this work, a plasmid production process was performed comparing conventional Erlenmeyer flasks with LB medium and the Thomson’s Ultra Yield® system. E. coli cells were cultivated in both systems in an orbital incubator shaker (Multitron, INFORS HT), bacterial growth was monitored, and the plasmids were purified at the end of cultivation. Subsequently, the quality of the isolated plasmids was analyzed by HPLC. The combination of the Ultra Yield® flasks with the enriched Plasmid+® medium and the AirOtop® enhanced seal led to higher cell densities and a 21-fold higher amount of plasmid than in Erlenmeyer shake flasks with LB medium.
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New User Protocol
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Removing adherently grown cells from a µ-Channel after cultivation
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Experimental setup optimization of wound healing assays and cell migration assays
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Methods for data analysis of wound healing assays and cell migration assays
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