NeoStress

Assay principle

The assay relies on the controlled generation of intracellular radical species by a photo-induction process. A cell permeant biosensor is added to the cell culture medium and binds to nucleic acids with a low fluorescence level. When biosensor is photoactivated by appropriate LED illumination, it’s relaxation is accompanied by an energy transfer to the intracellular dioxygen molecule (3O₂)  resulting in the production of singlet oxygen ( ¹O₂ ) which in turn triggers a cascade of Reactive Oxygen Species (ROS) production including the free radical species superoxide anion (O_2^*- ) and hydroxyl radical (OH* ). Presence of ROS species leads to cell alteration and fluorescence level increases. Neutralization of intracellular free radicals by sample added in the culture medium inhibits this process, maintaining fluorescence emission at low level. Kinetic records allow for antioxidant index calculation. Dose-response curves fitting with sigmoid model allow for efficacy standard concentrations (EC₅₀ , EC₁₀ , EC₉₀ ) evaluation (patented technology).

Features:  

• For 100 or  400 measure points in 96-well plates
• One-step procedure
• No washes
• Standard procedure to most cell lines (optimization kit available if needed)
• Adapted to cell lines, hiPSCs, primary cells,..
• 2 solutions in the kit (biosensor to be diluted in the culture medium and positive control).
• Storage 4°C ( Biosensor) and -20*C ( Control)
• Time to expiration; 6 months
• Time to expiration: 6 months after receipt
• Also available for 384-well plates

Protocol: 

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