ELISA

pre-coated ELISA

ELISA Kits I. Required material Reagents - A primary antibody detection, specific to the target molecule - A recombinante molecule to use as a standard - Detection Solutions: e.g. Streptavidin-HRP and TMB Buffers - Blocking Buffer: e.g. PBS with 5%BSA - Wash Buffer: e.g. PBS with 0.05%Tween - Diluent for Sample and Standard: e.g. PBS 1%BSA - Stop Solution: e.g. diluted H2SO4 Material - 96 well microtitre plates precoated with capture...

uncoated ELISA

I. Required material Reagents - A primary antibody capture, specific to the target molecule - A second primary antibody detection, specific to the target molecule - A recombinante molecule to use as a standard - Detection Solutions: e.g. Streptavidin-HRP and TMB Buffers - Coating Buffer: e.g. PBS - Blocking Buffer: e.g. PBS with 5%BSA - Wash Buffer: e.g. PBS with 0.05%Tween - Diluent for Sample and Standard: e.g. PBS 1%BSA - Stop Solution: e...