Detergent exchange mini spin columns
for rapid and effective removal of free detergents micelles and complete detergent exchange
Features :
- Rapid removal of free detergent micelles in 10 minutes
- Generate concentrated protein free of detergent micelles
- complete detergent exchange/removal
- Weak cation or anion exchanger
- High protein binding capacity (2mg)
- Simple load, wash and elute procedure
- Compatible with common lab instrumentation (only requires a microcentrifuge)
The Proteus Detergent Anion&Cation Exchange (DetEx) Mini Spin Columns are designed for rapid and effective removal of free detergents micelles and complete detergent exchange.
The D-Type anion exchange spin columns are optimized for membrane proteins with pI<7-8 in complex with non-ionic or zwitterionic detergents. The C-Type cation exchange spin columns are optimized for membrane proteins with pI >7-8.
Simple and adaptable to your protein requiring only a micofuge for operation. Faster and more effective than other methods of detergent removal or exchange such as chromatography (hydrophobic adsorption, ion exchange, affinity, gel filtration), dialysis and precipitation.
Ideal for applications such as ELISA, IEF, MS and NMR which suffer from interference with excess detergents.
Product code
Description
Size (columns)
Produit
PAL-MINI
PCL-MINI
Column type
D-Type
C-Type
Column bed volume
0.2 ml
0.2 ml
Membrane matrix
Stabilized regenerated cellulose
Stabilized regenerated cellulose
Nominal pore size
3-5 µm
3-5 µm
Amount of ionic groups
145-218 µEquivalents/ml for monovalent ions
72 µEquivalents/ml for monovalent ions
Membrane area
7.5 cm²
7.5 cm²
Working pH
4-10
4-10
Binding capacity
2 mg
2 mg
Membrane chemistry
Diethylamine ion exchange
Carboxyl weakly acidic cation exchanger
Approximate pKa or ionic groups
9.5
4.5
Maximum loading volume
0.4 ml (fixed angle rotor)
0.4 ml (fixed angle rotor)