Sanger Sequencing cleanup
Kits and reagents for Dye Terminator Removal
Performa® DTR – The gold standard in gel filtration based dye terminator removal!
Downstream Applications
Performa DTR Multi-Well Systems
Overview
The plates are aivalable in three formats :
Shortest Time to Completion
Performa® DTR Individual Cartridge Systems
Description
Performa DTR gel filtration cartridges are 800 µL spin columns assembled in 1.5 mL microcentrifuge tubes. These columns provide optimal performance for removal of unincorporated BigDye® v1.1, v3.0, and v3.1 and other dye terminators, dNTPs, salts, and other low molecular weight materials from sequencing reactions. These columns also remove DNA primers and fragments up to 20 bases, buffers, and nucleotides labeled with biotin, isotopes, and other assorted markers.
The spin columns are pre-packed with a fully hydrated matrix to afford optimal handling and performance characteristics. To minimize the potential for interference with sequencing applications, no preservatives, salts, or buffers are used in the preparation of these columns.
Performa® DTR utilizes a fully hydrated gel filtration matrix that provides a convenient and simple method of dye terminator removal for CE sequencing clean-up. Performa DTR ensures removal of BigDye® v3.1 or other dye terminators, along with dNTPs, salts and other low molecular weight materials from sequencing reactions. DNA primers and fragments up to 20 bases, buffers, nucleotides labeled with biotin, isotopes and other assorted markers are also eliminated. To minimize the potential for interference with sequencing applications, no preservatives, salts or buffers are used in the preparation of Performa DTR.
Features
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Downstream Applications
- DNA Sequencing
- SNP Genotypng
Performa DTR Multi-Well Systems
Overview
The plates are aivalable in three formats :
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| Performa DTR Ultra 96-Well Plates For a total reaction volume of 5-20 µl; for special sequencing protocols requiring high Dye Terminator volumes (also available as kit including lids and receiver plates, which can be purchased separately as well) |
Performa DTR V3 96-Well Short Plates For a total reaction volume of 5-15 µl (also available as kit including lids and receiver plates, which can be purchased separately as well) |
Performa DTR 384-Well Plates For a total reaction volume of 5-10 µl (only available without receiver plate, which can be purchased separately) |
Shortest Time to Completion
- Ready-to-Use: Pre-packed with a fully hydrated gel filtration matrix
- Simplest protocol with minimal handling
- Only 3 steps!
| Format | Size | Product code |  |
| Performa® DTR ultra | |||
| 96-Well plate | 2 plates | 54789 | |
| 10 plates | 55373 | ||
| 10 plates (no receiver) | 74521 | ||
| 50 plates (no receiver) | 86077 | ||
| 384-well plate | 2 plates (no receiver) | 52571 | |
| Performa® DTR V3 | |||
| 96-Well short plate | 2 plates | 63887 | |
| 10 plates | 89939 | ||
| 10 plates (no receiver) | 47938 | Plates Flowchart Collect purified products directly into ABI Prism MicroAmp® Optical Reaction Plate or comparable plates, load directly onto your capillary sequencing platform. |
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| 50 plates (no receiver) | 80808 | ||
Performa® DTR Individual Cartridge Systems
Description
Performa DTR gel filtration cartridges are 800 µL spin columns assembled in 1.5 mL microcentrifuge tubes. These columns provide optimal performance for removal of unincorporated BigDye® v1.1, v3.0, and v3.1 and other dye terminators, dNTPs, salts, and other low molecular weight materials from sequencing reactions. These columns also remove DNA primers and fragments up to 20 bases, buffers, and nucleotides labeled with biotin, isotopes, and other assorted markers.
The spin columns are pre-packed with a fully hydrated matrix to afford optimal handling and performance characteristics. To minimize the potential for interference with sequencing applications, no preservatives, salts, or buffers are used in the preparation of these columns.
| Size | Product code |  |
| Spin columns | ||
| 36/pk | 73328 | |
| 108/pk | 13266 | |
| Gel filtration Cartbridges | ||
| 36/pk | 98780 | |
| 108/pk | 42453 | Column Flowchart At the end of the procedure the sample is ready for immediate loading onto the capillary sequencing platform |