20% OFF on INDIGO Biosciences Assays Kits

Nuclear Receptor Assay Kits:INDIGO Biosciences offers a comprehensive portfolio of nuclear receptor assays, providing fast, accurate tools for evaluating chemical effects on nuclear receptors. These assays help researchers profile compounds, assess agonist and antagonist activity, and perform cross-species comparisons with ortholog kits

GPCR Assays: G-protein coupled receptors (GPCRs) are essential cell surface receptors that mediate extracellular signals to regulate cellular functions. Found throughout the body, they play key roles in processes like vision, smell, taste, and hormone response. Composed of seven transmembrane helices, GPCRs activate intracellular pathways via G-proteins upon ligand binding, influencing gene expression, enzyme activity, and more. As targets for many drugs, GPCRs are central to therapeutic research and innovation..

Luciferase Reporter Assays: INDIGlo Luciferase Detection Reagent from INDIGO Biosciences is a glow-type reagent optimized for measuring firefly and beetle luciferase expression in mammalian cells. Supplied as a 2x-concentrated kit with Luciferase Detection Reagent and Dilution Buffer, it supports both homogenous and non-homogenous assays. INDIGlo delivers a stable luminescence signal lasting over 2 hours with minimal fluctuation, making it ideal for cost-effective use in 96- to 1536-well plate formats.

Transcription Factor Assays: Transcription factors are key proteins that regulate gene expression by binding to specific DNA sequences in response to cellular signals. They control processes like development, immunity, and hormone signaling, making them essential for cellular adaptation and health. Understanding their function sheds light on their role in both physiology and disease.

Cell viability assays: The Live Cell Multiplex (LCM) Assay is a fluorescence-based method for quantifying live cells in assay plates, validating Nuclear Receptor Assay data. It detects if test compounds induce mitogenic, cytostatic, or cytotoxic effects on reporter cells, which is especially important in antagonist screens. This step prevents false-positive results caused by cytotoxic compounds that may falsely appear to inhibit nuclear receptor activity due to reduced luciferase signals.

Reporter-Based Assays

Cell-based assay kits

Proteins