This product contains TransStart^® FastPfu Fly DNA Polymerase, dNTPs and optimized reaction buffer at a concentration of 2×. It has the features of high amplification efficiency and fast amplification speed (≤ 5 kb fragments can achieve 12 kb/min extreme amplification, > 5 kb fragments can achieve 6 kb/min high-speed amplification). The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and Nuclease-free Water for amplification. The amplified product of 2×TransStart^®FastPfu Fly PCR SuperMix (-dye) can be cloned directly into pEASY^®-Blunt series of vectors. The amplified product of 2×TransStart^®FastPfu Fly PCR SuperMix (+dye) can be analyzed by electrophoresis directly, and need to be purified to remove dye when applied in cloning.

• Reduce PCR operation time.

•Avoid contamination caused by multi-step operation.

•TransStart® FastPfu Fly PCR SuperMix offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.

•Amplification of genomic DNA fragment up to 15 kb.

•Amplification of plasmid DNA fragment up to 20 kb.

Highlights

Fast, ultra-high fidelity, high specificity, good stability.

Applications

• Ultra high fidelity PCR

• Site-directed mutagenesis

• Blunt end cloning

• Complex templates

• GC/AT-rich templates

• Long fragment amplification

Storage
at -20 ℃ for two years

Shipping
Dry ice (-70 ℃)