25-500 bp DNA Marker

25-500 bp DNA Marker contains 12 DNA fragments ranging from 25 to 500 base pairs. The 100 bp and 200 bp bands have the highest intensity for easy identification. Ready-to-use, the blue dye in the loading buffer runs at approximately 4 bp on a 1% gel. Can optionally be used for mass determination. 1 test is equivalent to 1 lane.

Target	25-500 bp DNA Marker
Tested Applications	SDS-PAGE
Recommended dilutions	Recommended Agarose % (w/v) Effective Separation Range of Linear DNA (bp) 0.3 5000 - 60,000 0.5 1000 - 20,000 0.7 800 - 12,000 1.0 500 - 10,000 1.2 400 - 7000 1.5 200 - 3000 2.0 100 - 2000 2.5 50 - 100 3.0 10 - 75 Note: Circularized plasmids can be cut and linearized by an appropriate restriction enzyme to measure their molecular weight.
Form	Liquid
Storage	Store all components at -20 °C. Avoid repeated freeze/thaw cycles.
Validity	Up to 12 months.
Buffer	Contains a restriction digest plus one or more PCR products.
Availability	Shipped within 3-7 working days.
Note	This product is for research use only. Not for human consumption, cosmetic, therapeutic or diagnostic use.
Directions for use	Thaw, mix, and briefly centrifuge before use. Vortex the tube, then add 5 µl of ladder per lane. There is no need to add loading buffer to the ladder. The provided 5X Sample Loading Buffer can be used for sample loading; it should not be used for diluting the ladder.

Research Articles on 25-500 bp DNA Marker