Tetrazolium salt, abbreviated to MTT, is used for detection of dehydrogenases in combination with phenazine methosulfate (cat. no. 32030) (1). MTT is utilized as a colorimetric metabolic activity indicator in cell viability assays and to determine cell proliferation (3, 4). It works as a terminal electron acceptor in the cycling assay for pyridine nucleotides (2). MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm.

Assay (HPLC)

min. 98.0 %

References:

1. Schauenstein, E. & Höfler-Bergthaler, E. (1972) Monatsh. Chem. 103, 1271-5
2. Bernofsky, C. & Swan, M. (1973) Anal. Biochem. 53, 542-8
3. Gerlier, D. & Thomasset, N. (1986) J. Immunol. Methods 94, 57-64
4. Hansen, M.B. et al. (1989) J. Immunol. Methods 119, 203-10

WARNING

Hazard Statements	H315 - H319 - H335 - H341
Precautions	P261 - P280
Reaction	P302 +P352 - P304 +P340 - P305 +P351 +P338 - P337 +P313

Muta. 2 • EINECS: 206-069-5 • WGK: 2L • HS: 29341000
Storage Temperature: +15 °C to +30 °C