Alkaline phosphatase (ALP) is a hydrolase enzyme that removes phosphate groups from nucleotides proteins and alkaloids. Changes in ALP level and activity are associated with various disease states in the bone and liver. ALP is also a common enzyme conjugated to secondary antibody used in ELISA.

 The Alkaline Phosphatase Activity Fluorometric Assay Kit provides a highly sensitive and convenient way for detection of ALP activity based on fluorometric method in serum and biological samples. In the assay, ALP removes the phosphate group of the non-fluorescent 4-Methylumbelliferyl phosphate disodium salt (MUP) substrate producing an intense fluorescent signal (Ex/Em = 360 nm/440 nm). The kit is an ultra sensitive, HTS-ready assay that is more sensitive than colorimetric method with detection sensitivity ~1 µU. The assay is suited for both research and drug discovery.